聚磺酸甜菜碱功能化金纳米粒子的抗蛋白吸附

Resistance Protein Adsorption Property of Poly-Sulfobetaine Methaerylate Functional Gold Nanoparticles

针对金纳米粒子(GNPs)在体内吸附蛋白质从而引起生物相容性的问题,通过两性离子聚合物表面修饰来提高金纳米粒子的抗蛋白吸附性能。通过可逆加成断裂链转移聚合(RAFT)制备了聚磺酸甜菜碱(PSBMA),并利用巯基与金之间的强耦合作用获得了PSBMA修饰的金纳米粒子PSBMA-@-GNPs。利用UVvis光谱、动态光散射(DLS)和透射电镜(TEM)证实了PSBMA-@-GNPs的核-壳纳米结构。以牛血清白蛋白(BSA)为蛋白模型,对比了GNPs、PEG-@-GNPs和PSBMA-@-GNPs对BSA的吸附性能。研究结果表明:由于表面PSBMA特殊的聚合物长链和两性离子结构,大大降低了GNPs的蛋白吸附。

In order to solve the problem that protein-adsorbing capacities of gold nanoparticles( GNPs) caused the poor biocompatibility in vivo,anti fouling properties of GNPs were increased by surface coating of zwitterionic polymer. Poly-sulfobetaine methaerylate( PSBMA) was prepared by reverslable addtion fragmentation chain transfer pdymerization( RAFT),and then was coated on the surface of GNPs to prepare PSBMA-@-GNPs via the strong binding between the thiol end-functionalization of polymer and gold. The coreshell structure of PSBMA-@-GNPs was determined by UV-vis spectra,dynamic light scattering( DLS) and transmission electron microscopy( TEM). Taking bovine serum albumin( BSA) as a protein model,the protein absorption of GNPs,PEG-@-GNPs and PSBMA-@-GNPs was investigated. The results indicate that the protein absorption of GNPs is greatly reduced by PSBMA modification due to its long-chain structure and zwitterionic property.