一种含有富里酸及腺苷的葛花解酒产品的研制

Development of a Kind of Pueraria lobata Flowers Anti-inebriation Product Containing Fulvic Acid and Adenosine

富里酸腺苷、葛花提取液与富里酸复配解酒产品,具有解酒和保健的双重功效。采用微波和超声波协同提取葛花水溶物;并将从富里酸提取液中筛选出的有益菌-富里酸拟青霉菌进行培养,采用微波和超声波协同提取富里酸拟青霉菌中的腺苷,利用高效液相色谱法进行鉴定,得出腺苷含量;将葛花提取液、富里酸及拟青霉菌腺苷提取液进行复配,制备解酒产品。通过醉酒小鼠"翻正反射"实验,证明产品的解酒防醉功效,获得最佳的复配条件为葛花提取液∶拟青霉菌腺苷提取液∶富里酸=1∶2∶4(体积比),其中葛花提取液浓度为5.872 g/L,腺苷的浓度为0.4 g/L;富里酸的浓度3 g/L。

There has the dual function of detoxifying wine and health care for the mixed anti-inebriation product containing fulvic acid, adenosine and Pueraria Iobata flowers. Firstly, water soluble content of Pueraria Iobata flowers were extracted by microwave and ultrasonic. Secondly, the beneficial bacteria-fulvic acid paecilomyces penicillium selected from the fulvic acid extract were cultivated, and the adenosine was extracted by microwave and ultrasonic from fulvic acid paecilomyces penicillium. The adenosine content was confirmed and determined by high performance liquid chromatography （HPLC）. Finally, the extract of Pueraria Iobata flowers, fulvic acid and paecilomyces penicillium adenosine extracting solution were mixed to develop the anti-inebriation products. The effect of the product was proved through the drunken mice ＂righting reflex＂ experiment, and the best compound conditions were obtained. The optimal compound condition was the extract of Pueraria lobata flowers ： the extract of adenosine of pseudomycelia penicillium ： fulvic acid = 1 ： 2 ： 4 （volume ratio）, in which the extract of Pueraria Lobata flowers was 5.872 g/L, the concentration of adenosine was 0.4 g/L and the concentration of fulvic acid was 3 g/L.