低温处理不同恢复时间高粱幼苗渗透调节相关指标的变化

Variations of Osmotic Adjustment-related Indexes in Different Recovery Time of Sorghum Seedlings Treatedat Low Temperature

为揭示低温对高粱幼苗生长的影响,以高粱龙杂17为研究对象,对三叶一心期高粱幼苗进行5℃低温处理,分别于恢复1、3、5和7d取样测定生理生化指标。结果表明:与未处理对照相比,除低温恢复1d的高粱幼苗根冠比显著抑制外,其余处理均表现为显著增加;高粱幼苗的株高和地上鲜重变化在供试时间点均表现为显著抑制。供试时间点脯氨酸和可溶性糖含量基本都增加,其中恢复1和3d的高粱幼苗脯氨酸含量显著增加,恢复3d的高粱幼苗可溶性糖含量显著增加。高粱幼苗TBARS含量也在整个供试期增加,在恢复3和5d显著增加;但过氧化氢含量变化不显著。上述结果表明,低温处理造成了生物膜不同程度的损伤,脯氨酸和可溶性糖等渗透调节在高粱幼苗耐冷的过程中发挥重要作用。

In order to reveal the effects of low temperature on the growth of sorghum seedlings,the three-leaf with one new leaf sorghum Longza 17 seedlings were treated with low temperature at 5 ℃,and they were restored and collected at 1,3,5 and 7 d,respectively.The indicators of growth and physiological and biochemical function were analyzed.The results showed that compared with the untreated controls,except for the rootshoot ratio of sorghum seedlings under 1 drecovered was significant suppression,the other treatments showed a significant increase;the plant height and fresh weight of above ground of sorghum seedlings were significantly inhibited at the time tested.The contents of proline and soluble sugar always increased.Among them,the proline content significantly increased at 1 and 3 d,and the content of soluble sugar significantly increased at 3 d.The contents of TBARS in sorghum seedlings also increased during the whole period tested,and significant increase showed at 3 and 5 d.However,the contents of hydrogen peroxide remained unchanged significantly.The above results indicated that low temperature treatment caused the various degree damage of membranes,and osmotic adjustment materials such as proline,played an important role in tolerance to low temperature of sorghum seedlings.