白芨无菌小苗一步成苗培养基优化

Optimization of Mediums on One-step Culture of Bletilla striata Aseptic Seedlings

为提高白芨组织培养效率,以白芨种子播种的无菌小苗为外植体,利用正交设计法研究了培养基中6-BA(A)、NAA(B)、香蕉泥(C)和马铃薯提取液(D)4种因素对株高和假鳞茎粒径的影响。结果表明:4个因素对株高影响的主次关系为B>A>D>C,对假鳞茎粒径影响的主次关系为A>D>B>C;无菌小苗一步成苗的最佳培养基配方是1/2 MS+1.0mg·L^(-1)6-BA+1.0mg·L^(-1) NAA+50g·L^(-1)香蕉泥。

In order to improve the tissue culture efficiency of Bletilla striata ,taking aseptic seedlings of Bletilla striata as explants,the orthogonal design was used to study the effects of 6-BA（A）,NAA（B）,mashed banana（C） and potato extract（D） in the culture medium on the plant height and pseudobulb diameter of Bletilla striata .The results indicated that the primary and secondary effects of the four factors on plant height were B〉A〉 D〉C and the effect on pseudobulb diameter were A〉D〉B〉C.The optimal medium of one-step culture of Bletilla striata aseptic seedlings was 1/2 MS＋1.0 mg ·L -1 6-BA＋1.0 mg ·L -1 NAA＋50 g ·L -1 mashed banana.