电针百会印堂穴对新生大鼠七氟烷麻醉苏醒期谵妄躁动的影响

The effects of electroacupuncture at Baihui and Yintang acupoints on delirium and emergence agitation in neonatal rats after sevoflurane exposure

目的探讨电针百会、印堂穴对新生大鼠七氟烷麻醉苏醒期谵妄躁动的作用和影响。方法选取生后7天雄性Sprague-Dawley大鼠50只,按随机数字表法分为五组,即七氟烷麻醉组(n=10)、七氟烷假针组(n=10)、电针1组、电针2组及电针3组(n=10)。电针1~3组分别于麻醉前、中、后针刺百会、印堂并持续给予恒定电流30 min,假针组进行安慰治疗。观察记录新生大鼠麻醉苏醒期间躁动谵妄的发生时间、频次及强度,记录各组麻醉苏醒时间。结果与七氟烷麻醉组及假针组比较,电针1组、电针2组在苏醒期发生谵妄躁动的频次、强度及时间及苏醒时间差异均无统计学意义(P>0.05),而电针3组在苏醒期发生谵妄躁动的时间、频次、强度及苏醒总时间有统计学意义(P<0.05)。结论麻醉后采用电针百会印堂穴的方法可明显降低新生大鼠七氟烷麻醉苏醒期谵妄躁动发生频率,并且可显著增加苏醒时间,在麻醉后苏醒期可起到较好的安神镇静作用。

Objective To explore the effects of electroacupuncture at Baihui and Yintang acupoints on delirium and emergence agitation in neonatal rats after sevoflurane exposure.Methods Fifty male Sprague-Dawley rats aged 7 days were randomly divided into five groups of sevoflurane,pre-,middle-and post-anesthesia electroacupuncture and pseudo-acupuncture,10 in each group. Electroacupuncture treatment with constant current was given for 30 min to the animals except pseudo-acupuncture group which received placebo treatment.The frequency,time and intensity of delirium and emergence agitation in the post-anesthesia rats were observed and the awakening time was recorded.Results Compared to the groups of sevoflurane and pseudo-acupuncture,there was no statistical difference in the frequency,time and intensity of delirium and emergence agitation in the groups of pre-and middle-anesthesia electroacupuncture（ P〈0. 05）,however,the differences were statistically significant in the post-anesthesia electroacupuncture group（ P〈0. 05）.Conclusion Post-anesthesia electroacupuncture can significantly reduce the occurrence of delirium and emergence agitation,and increase the awaking time in rats after neonatal sevoflurane exposure.Therefore,it plays an important role in sedation during awaking time.