HPV58型E6蛋白B细胞表位预测与分析

Prediction and analysis of B cell epitopes of type HPV58 E6 protein

目的:预测分析高危型人乳头瘤病毒(HPV)58型E6蛋白的B细胞线性表位及其结构。方法:从瑞士生物信息研究所提供的Swissprot蛋白质数据库中检索到E6蛋白序列,采用计算机辅助生物信息学软件进行分析,用Expasy服务器在线软件分析预测E6蛋白的理化性质、二级结构及三维空间结构,采用DNASTAR软件包中的protean软件进一步分析亲水性、柔韧性、表面可及性、抗原性与极性等特性,再结合氨基酸抗原指数(AI)计算法算出平均AI,根据AI大小预测HPV58型E6蛋白的B细胞线性表位的可能肽段,并进行同源性匹配分析。结果:HPV58型E6蛋白的B细胞线性表位可能位于以下肽段位置:N端8~15(KPRTLHDL)、27~44(ELKCVECKKTLQRSEVYD)、108~127(RPLCPQEKKRHVDLNKRFHN)、141~147(RPRRRQT)肽段及其周围。同源性分析得出肽段27~44、141~147为可能的B细胞线性表位,为HPV58型E6蛋白B细胞表位的独特序列,肽段8~15与HPV33型E6蛋白同源性匹配为100%,108~127与HPV33型E6、HPV9型E6蛋白同源性匹配为100%。结论:27~44、141~147肽段为可能的HPV58型E6蛋白的B细胞优势表位。

Objective： To predict and analyze the B cell linear epitopes of the high-risk human papillomavirus HPV 58 E6 protein and its structure. Methods： E6 protein sequences retrieved from the Swissprot protein database provided by the Swiss Institute of bioinformatics were analyzed using computer aided bioinformatics software, and Expasy Server analysis was used to predict physicochemical properties, secondary structure,three-dimensional structure of E6 protein. DNASTAR software was used to further analyze its hydrophilicity,flexibility, surface probability, antigenicity and polarity and other characteristics. Combined with the amino acid antigen index calculation method, the average antigen index（AI） was calculated, and according to the AI size, the potential peptide of B cell linear epitopes of HPV 58 E6 protein was predicted and analyzed by homology matching.Results： The B cell linear epitopes of HPV58 E6 protein may be located at the following peptide position：N-terminal 8-15（KPRTLHDL）, 27-44（ELKCVECKKTLQRSEVYD）, 108-127（RPLCPQEKKRHVDLNKRFHN）,141-147（RPRRRQT） peptide and its surrounding. The homology analysis showed that peptide 27-44 and141-147 are possible linear epitopes of B cells, which are unique sequences of cell type HPV58 E6 B epitopes.The homology match between peptide 8-15 and HPV33 type E6 protein was 100%. The peptide 108-127 matches HPV33 type E6 and HPV9 E6 protein being 100% homologous. Conclusion： 27-44, 141-147 peptide are the potential dominant epitopes of B protein of HPV58 type E6.