人参皂苷Rg1对NAFLD动物模型中同型半胱氨酸的调控机制

Regulatory Mechanism of Ginsenoside Rg1 on Homocysteine in NAFLD Model

目的建立非酒精性脂肪性肝病(NAFLD)的动物模型,探讨人参皂苷Rg1调控同型半胱氨酸的分子机制.方法建立NAFLD动物模型,测定正常对照组和模型对照组空腹状态下的血清甘油三酯(TG)和总胆固醇(TC),HE染色检测肝脏的形态学改变,油红"O"染色检测肝脏的脂质沉积。各组取血清检测同型半胱氨酸(Hcy)含量,免疫组化和Western blot检测肝脏组织内亚甲基四氢叶酸还原酶(MTHFR)和胱硫醚合成酶(CBS)蛋白表达的变化.结果与正常对照组比较,模型对照组空腹TG和TC浓度增加(P<0.05);HE染色显示肝细胞排列紊乱,出现脂肪变性;油红"O"染色显示肝脏组织的脂质沉积增加.与正常对照组比较,模型对照组的Hcy的浓度增加(P<0.05),人参皂苷Rg1治疗后降低(P<0.05),存在剂量依赖性;与正常对照组比较,模型对照组的MTHFR和CBS的蛋白质在肝脏组织中表达降低(P<0.05).人参皂苷Rg1治疗后,MTHFR和CBS的蛋白质表达升高(P<0.05),存在剂量依赖性.结论在NAFLD模型中,人参皂苷Rg1可能通过增加肝脏组织的MTHFR和CBS表达,降低同型半胱氨酸的含量,从而对非酒精性脂肪肝病发挥保护作用.

Objective To establish animal model of non-alcoholic fatty liver disease（NAFLD） and explore the molecular mechanism of homocysteine changes in SD rats with NAFLD by ginsenoside Rg1 treating. Me thods NAFLD animal model was established to determine serum triglyceride and total cholesterol in the fasting state. HE staining was used to detect the morphological changes of the liver,and lipidosis of the liver tissue was observed by the oil red ＂o＂ staining in the control group and model control group. The serous concentration of homocysteine wasmeasured in every group by ELISA. Immunohistochemistry and Western blot were used to detect the expression levels of methylenetetrahydrofolate reductase（MTHFR） and cystathionine synthetase（CBS） in liver tissues. Results The concentrations of triglyceride and total cholesterol in the model control group were disordered compared with the control group（P0.05）,meanwhile the morphology of the liver tissue was disordered and lipidosis was obvious in the model control group. So those results indicated the NAFLD model was successful. The concentration of homocysteine in the model control group was increased and decreased by Ginsenoside Rg1 treating（P0.05）. The protein expressions of MTHER and CBS in model control group were lower compared with the control group by immunohistochemistry and Western blot（P0.05）. The protein expressions ofMTHFR and CBS in the ginsenoside Rg1 treatment group were higher compared with the model control group by immunohistochemistry and Western blot（P0.05）. The results have dose-dependent. Conclusion Ginsenoside Rg1 is possible to increase the protein expressions of MTHFR and CBS and decrease the concentration of homocysteine in animal models of non-alcoholic fatty liver, thus it can play a protective effect in non-alcoholic fatty liver.