芦丁对瘢痕疙瘩成纤维细胞增殖的影响

Effects of Rutin on Proliferation of Keloid Fibroblasts

目的探讨芦丁对瘢痕疙瘩成纤维细胞的增殖作用.方法应用瘢痕疙瘩组织诱导成纤维细胞;芦丁分为0、50、100、200、300μmol/L等5个浓度梯度,通过CCK-8及Ed U实验观察芦丁对瘢痕疙瘩成纤维细胞增殖的影响,通过Western blot检测芦丁对TGF-β/Smad信号通路的影响.结果当浓度低于300μmol/L时,芦丁对瘢痕疙瘩组织来源成纤维细胞增殖产生明显抑制作用(P<0.01),并且呈剂量依赖性;Ed U实验结果显示,瘢痕疙瘩组织来源成纤维细胞DNA合成受到芦丁的抑制作用;50、100、200μmol/L条件下,芦丁可以明显地抑制瘢痕疙瘩组织来源成纤维细胞TGF-β1及Smad2的表达.结论芦丁通过调控TGF-β/Smad信号通路抑制瘢痕疙瘩成纤维细胞的增殖.

Objective To investigate the effects of rutin on the proliferation of keloid fibroblasts.Methods Keloid tissue was used to induce fibroblasts and 5 concentration gradients of rutin were set： 0,50,100, 200 and300 μmol/L.The effects of rutin on the proliferation of keloid-derived fibroblasts were observed by CCK-8 and Ed U assays.Western blot was applied to detect the effect of rutin on TGF-β/Smad signaling pathway.Re s ults Rutin inhibited the proliferation of keloid-derived fibroblasts in a dose-dependent manner when concentration gradient was lower than 300 μmol/L（P〈0.01）. The result of Ed U assay showed that the synthesis of DNA in keloid-derived fibroblasts was inhibited by rutin. At the concentration gradient of 50, 100 and 200 μmol/L, rutin could significantly inhibit the expression of TGF-β1 and Smad2 in keloid-derived fibroblasts.Conclus ion Rutin inhibits the proliferation of keloid-derived fibroblasts by regulating TGF-β/Smad signaling pathway.