不同浓度二甲双胍对猪精液液态保存效果的影响

Effects of Different Concentrations of Metformin on the Liquid Storage of Boar Sperm

[目的]研究不同浓度二甲双胍对猪精液液态保存效果的影响。[方法]在猪精液液态保存液中分别添加不同浓度(0.5、1.0、2.0、4.0 mmol/L)的二甲双胍,测定不同保存时间精子的活力、活率、质膜完整率、顶体完整率等指标。[结果]猪精液液态保存第3天,在不同浓度二甲双胍作用下各组间精子活力出现差异。第5天,添加1.0 mmol/L二甲双胍的保存液保存的精子活力达到38.03%,精子活率为49.03%,质膜完整率为43.91%,顶体完整率为50.88%,均高于对照组。[结论]添加二甲双胍有助于提高液态保存猪精液的质量,延长保存时间。

[Objective] To study the influences of different concentrations of metformin on the liquid storage effect of boar sperm.[Method]Different concentrations（ 0.5,1.0,2.0,4.0 mmol/L） of metformin was added in the liquid storage liquid of boar sperm to detect the sperm motility,viability,plasma membrane integrity rate and acrosome integrity rate and other indices.[Result]On the third day,there was difference of sperm motility among different groups with different concentrations of metformin.On the 5 thday,the sperm motility stored in the preservation liquid with adding 1.0 mmol/L metformin reached 38.03%,and the sperm viability was 49.03%,and plasma membrane integrity and acrosome integrity were43.91% and 50.88% respectively,which were all higher than those of control group.[Conclusion]Metformin was beneficial to improve the quality of boar semen in liquid storage and prolong the preservation time.