摘要
[目的]研究不同浓度二甲双胍对猪精液液态保存效果的影响。[方法]在猪精液液态保存液中分别添加不同浓度(0.5、1.0、2.0、4.0 mmol/L)的二甲双胍,测定不同保存时间精子的活力、活率、质膜完整率、顶体完整率等指标。[结果]猪精液液态保存第3天,在不同浓度二甲双胍作用下各组间精子活力出现差异。第5天,添加1.0 mmol/L二甲双胍的保存液保存的精子活力达到38.03%,精子活率为49.03%,质膜完整率为43.91%,顶体完整率为50.88%,均高于对照组。[结论]添加二甲双胍有助于提高液态保存猪精液的质量,延长保存时间。
[Objective] To study the influences of different concentrations of metformin on the liquid storage effect of boar sperm.[Method]Different concentrations( 0.5,1.0,2.0,4.0 mmol/L) of metformin was added in the liquid storage liquid of boar sperm to detect the sperm motility,viability,plasma membrane integrity rate and acrosome integrity rate and other indices.[Result]On the third day,there was difference of sperm motility among different groups with different concentrations of metformin.On the 5 thday,the sperm motility stored in the preservation liquid with adding 1.0 mmol/L metformin reached 38.03%,and the sperm viability was 49.03%,and plasma membrane integrity and acrosome integrity were43.91% and 50.88% respectively,which were all higher than those of control group.[Conclusion]Metformin was beneficial to improve the quality of boar semen in liquid storage and prolong the preservation time.
作者
周佳勃
马思楠
王娜
李文倩
聂云
邵千朔
王启蒙
岳顺利
ZHOU Jia-bo;MA Si-nan;WANG Na(Key Laboratory of Animal Cellular and Genetic Engineering of Heilongjiang Province,NortheastAgricultural University,Harbin,Heilongjiang 150030)
出处
《安徽农业科学》
CAS
2018年第20期77-79,82,共4页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金面上项目(31372311)
黑龙江省动物细胞与遗传工程重点实验室年度开放课题(KF201701)
东北农业大学2018年大学生创新训练(SIPT)项目(201810224072)
关键词
猪精液
液态保存
二甲双胍
活力
Boar sperm
Liquid storage
Metformin
Motility