阿拉善戈壁双峰驼的微卫星多态性与体尺性状关联分析

Microsatellite Polymorphism and Its Correlation Analysis with Body Size Traits in Alashan Gobi Bactrian Camel

本研究旨在利用微卫星标记分析阿拉善戈壁双峰驼的遗传多样性,寻找与其体尺性状相关联的微卫星标记,为阿拉善戈壁双峰驼的标记辅助选择提供参考。试验利用非变性聚丙烯酰胺凝胶电泳技术,检测17个微卫星标记在阿拉善戈壁双峰驼群体中的多态性,并与其体尺性状进行相关性分析。结果显示,17个微卫星标记共检测到60个等位基因,平均有效等位基因数为2.7302,平均杂合度为0.6205,平均多态信息含量为0.5308,可见阿拉善戈壁双峰驼群体的遗传多样比较丰富。微卫星标记多态性与阿拉善戈壁双峰驼体尺性状的关联分析发现,有8个微卫星标记(LCA82、LCA90、CMS15、CMS36、CMS104、CVRL01、YWLL36和YWLL44)与不同体尺性状有一定的关联性,尤其是CMS36和YWLL44与所测体尺性状均有关联,其中CMS36不同基因型的体长和体重均有显著差异(P<0.05),具体表现为BB>AA>AB,AA和BB基因型的体高、胸围、管围均显著高于AB基因型(P<0.05);YWLL44的AC基因型体高、胸围和体重均显著高于BD基因型(P<0.05),AC基因型的体长显著高于AB和BD基因型(P<0.05),AB和AC基因型的管围显著高于BD基因型(P<0.05)。研究表明,8个微卫星标记对阿拉善戈壁双峰驼体长有一定影响,CMS36和YWLL44对其体高有影响,LCA82、LCA90、CMS36、CVRL01、YWLL36和YWLL44对其胸围有影响,LCA82、CMS15、CMS36、CMS104、CVRL01和YWLL44对其管围有影响,LCA82、LCA90、CMS15、CMS36、CVRL01和YWLL44对其体重有影响。

This study was aimed to analyze the genetic diversity of Alashan Gobi Bactrian camel using microsatellite markers,find the microsatellite markers which associated with body size trait,and provide reference for marker-assisted selection of Alashan Gobi Bactrian camel.17 microsatellite markers were selected to detect the polymorphism in Alashan Gobi Bactrian camel population by non-denaturing polyacrylamide gel electrophoresis technique,and the correlation analysis was performed between microsatellite markers and body size traits.The results showed that 60 alleles were detected in 17 microsatellite markers,the average effective allele number was 2.7302,the average heterozygosity was 0.6205,the average polymorphic information content was 0.5308,which indicated that the genetic diversity of the Alashan Gobi Bactrian camel population was rich.The correlation analysis results indicated that 8 microsatellite markers（LCA82,LCA90,CMS15,CMS36,CMS104,CVRL01,YWLL36 and YWLL44）were associated with different body size traits,especially CMS36 and YWLL44,which were related to all body size traits.For CMS36 marker,there was significant difference between different genotypes and body length（P0.05）,the order was BBAAAB,the body height,chest circumference and cannon circumference of AA and BB genotypes were significantly higher than that of AB genotype（P0.05）.For YWLL44 marker,the body height,chest circumference and body weight of AC genotype were significantly higher than that of BD genotype（P0.05）,the body length of AC genotype was significantly higher than that of AB and BD genotypes（P0.05）,and the chest circumference of AB and AC genotypes was significantly higher than that of BD genotype（P0.05）.Summarily,8 microsatellite markers including LCA82,LCA90,CMS15,CMS36,CMS104,CVRL01,YWLL36 and YWLL44 had certain effects on the body length of Alashan Gobi Bactrian camel,CMS36 and YWLL44 had influence on the body height;LCA82,LCA90,CMS36,CVRL01,YWLL36 and YWLL44 had influence on the chest circumference;LCA82,CMS15,CMS36,CMS104,CRVL01 and YWLL44 had influence on the cannon circumference;LCA82,LCA90,CMS15,CMS36,CVRL01 and YWLL44 had influence on the body weight.