高、低产双黄蛋高邮鸭卵巢组织差异分析

Differential Analysis of Ovary Structure Between High and Low Double-yolk Egg Laying Gaoyou Duck

本试验以高邮鸭为研究对象,选择双黄蛋高、低产组高邮鸭各6只,颈动脉放血致死后立即采集下丘脑、垂体、肝脏、输卵管及卵巢组织,将采集的卵巢组织通过组织切片、HE染色观察卵巢形态结构,并对双黄蛋高、低产组高邮鸭卵巢中等级卵泡进行统计对比;进行特异性标记蛋白卵泡刺激素受体(FSHR)免疫组化染色,观察两组高邮鸭卵巢阳性细胞表达情况及卵巢颗粒细胞分布;同时提取下丘脑、垂体、肝脏、输卵管及卵巢组织RNA,采用实时荧光定量PCR技术检测FSHR基因的表达。结果显示,双黄蛋高产和低产组高邮鸭卵巢在组织结构及卵泡发育上存在明显差异,高产组卵巢上的卵泡繁密且发育良好,密布着众多小黄卵泡、大白卵泡及小白卵泡,各级卵泡大小差异明显;而低产组卵巢上的卵泡稀疏且发育较为迟缓,小黄卵泡、大白卵泡及小白卵泡数量较少。实时荧光定量PCR检测结果表明,高产组高邮鸭下丘脑、垂体、肝脏及输卵管中的FSHR基因表达量极显著高于低产组(P<0.01),而卵巢中FSHR基因表达量在两组中差异不显著(P>0.05)。本试验结果表明,双黄蛋高、低产组高邮鸭的卵巢结构及卵泡发育存在显著差异,卵巢上的微环境可能是影响高邮鸭双黄蛋产蛋性能的影响因素。

Gaoyou ducks were used as the experiment objects in this study,12 Gaoyou ducks were chosen from high and low double-yolk egg laying groups（6 ducks per group）,and hypothalamus,pituitary gland,liver,fallopian tube,ovary tissues were collected immediately after bleeding to death by carotid artery cut,HE staining was made to observe the ovary morphological structure and compare the development levels of follicle in two groups.FSHR immunohistochemical staining was conducted to observe its expression in positive cells and the distribution of ovarian granulosa cells.The total RNA was extracted from the hypothalamus,pituitary gland,liver,fallopian tube and ovary tissues,Real-time PCR method was used to detect the expression of FSHRgene in these tissues.The results showed that there were significant differences in ovary structure be-tween high and low laying Gaoyou ducks.The follicles were dense and well developed,numerous small yellow follicles,small and big white follicles were distributed,and the sizes of the follicles at all levels were significantly different in high laying Gaoyou ducks＇ ovary;While low laying Gaoyou ducks＇ ovary had less and undeveloped follicles.The results of Real-time PCR showed that the expression of FSHRgene in hypothalamus,hypophysis gland,liver and fallopian tube of high laying ducks were extremely significantly higher than those in the low laying ducks（P0.01）,but there were no significant difference in ovary between two groups（P0.05）.In conlusion,the ovary structure and follicle development of high and low laying Gaoyou ducks had significant differences,and the microenvironment in the ovary might affect double-yolk egg laying performance of Gaoyou duck.