荒漠肉苁蓉苯乙醇苷对酒精诱导的慢性肝损伤的修复作用(英文)

Hepatoprotective Effect of Phenylethanoid Glycosides from Cistanche deserticola against Chronic Hepatic Injury Induced by Alcohol

为了研究荒漠肉苁蓉对酒精诱导的慢性肝损伤的修复效果及其作用机制,从荒漠肉苁蓉中提取并纯化苯乙醇苷。抗氧化活性研究结果表明,苯乙醇苷具有显著的抗氧化活性。另外,分别通过体外和体内实验研究了苯乙醇苷的肝保护活性。在体外实验中,苯乙醇苷可显著提高Hep G2细胞的存活率。对酒精诱导的肝损伤小鼠模型进行动物体内实验,结果表明苯乙醇苷可显著恢复由酒精诱导的血清指标(丙氨酸氨基转氨酶、天冬氨酸转氨酶、γ-谷氨酰转肽酶、酸性磷酸酶、甘油三酯和低密度脂蛋白胆固醇)和肝脏生化指标(超氧化物歧化酶、谷胱甘肽S-转移酶、谷胱甘肽、谷胱甘肽过氧化物酶、丙二醛和甘油三酯)水平。模型动物的肝组织显微结构中,突出的病理学脂肪微泡和细胞坏死情况也由于灌胃苯乙醇苷而显著减弱。这些结果表明,针对酒精诱导的慢性肝损伤,苯乙醇苷具有显著的肝保护活性。所述机制涉及对相应酶(包括超氧化物歧化酶、谷胱甘肽S-转移酶、谷胱甘肽过氧化物酶)活性以及对脂质过氧化物(丙二醛)的调节。

The aim of this paper is to explore the hepatoprotective effect of Herba Cistanchis against alcohol-induced chronic hepatic injury and its underlying mechanism. Phenylethanoid glycosides（Ph Gs） were extracted and purified from Cistanche deserticola. Then, the Ph Gs composition was analyzed and the antioxidant activity was determined. The hepatoprotective activity was studied both in vitro and in vivo. In vitro, the survival of Hep G2 cells was promoted by Ph Gs. In vivo, Ph Gs restored the alterations induced by alcohol, including serological indexes（alanine aminotransaminase, aspartate aminotransferase, γ-glutamyltranspeptidase, acid phosphatase, triglyceride and low density lipoprotein-cholesterol） and hepatic indicators（superoxide dismutase, glutathione S-transferase, glutathione, glutathione peroxidase, malondialdehyde and triglyceride） levels. The prominent microvesicular steatosis and necrosis in hepatic histopathology of model animals were also notably attenuated by Ph Gs administration. These results indicated that Ph Gs possess hepatoprotective properties against chronic alcohol-induced liver injury, the mechanisms involve the modulation of related enzyme（including superoxide dismutase, glutathione S-transferase, glutathione peroxidase） activities and the reduction of lipid peroxidation products such as malondialdehyde.