植物乳杆菌C88对高脂饲料和链脲佐菌素诱导2型糖尿病模型大鼠的降血糖作用

Antidiabetic Effect of Lactobacillus plantarum C88 in a Rat Model of High-Fat Diet and Streptozotocin-Induced Type 2 Diabetes

目的:探究植物乳杆菌(Lactobacillus plantarum)C88对2型糖尿病大鼠模型降血糖作用。方法:采用高脂饲料结合腹腔注射链脲佐菌素的方法建立2型糖尿病大鼠模型,连续灌胃植物乳杆菌C88,28 d后检测大鼠空腹血糖量、葡萄糖耐受量、血脂、胰岛素、炎症因子、肠道屏障功能和菌群水平。Western blot分析核因子-κB(nuclear factor-κB,NF-κB)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、闭锁小带蛋白-1(zonula occludens-1,ZO-1)、occludin、claudin-1的表达。结果:植物乳杆菌C88可有效改善2型糖尿病大鼠高血糖和血脂代谢紊乱;显著降低2型糖尿病大鼠血清中TNF-α、白细胞介素-6、C反应蛋白、脂多糖、D-乳酸等炎症相关因子水平;植物乳杆菌C88能促进胰岛素的分泌,提高胰高血糖素样肽-2水平,降低2型糖尿病大鼠血清中二胺氧化酶和连蛋白水平;显著降低肝脏中炎症相关蛋白NF-κB、TNF-α的表达,提高回肠中肠道通透性相关蛋白ZO-1、occludin、claudin-1的表达。结论:植物乳杆菌C88是一株具有辅助降血糖功能的益生菌。

Objective： To investigate the antidiabetic effect of Lactobacillus plantarum C88 in a type 2 diabetic rat model. Methods： Type 2 diabetes was induced in rats by feeding high-fat diet followed by single intraperitoneal injection of streptozotocin. The diabetic rats were intragastrically administrated with L. plantarum C88 for 28 consecutive days, and then the fasting blood-glucose, glucose tolerance, blood lipids, insulin, inflammatory factors, intestinal barrier function and intestinal flora were measured. Western blot was used to analyze the expression of nuclear factor-κB（NF-κB）, tumor necrosis factor-α（TNF-α）, zonula occludens-1（ZO-1）, occludin and claudin-1. Results： L. plantarum C88 could effectively improve hyperglycemia and lipid metabolism disorder in type 2 diabetic rats and significantly decrease the levels of TNF-α, interleukine-6, C-reactive protein, lipopolysaccharide, D-lactic acid and other inflammatory factors in the serum.L. plantarum C88 could promote the secretion of insulin, increase glucagon-like peptide-2 level and decrease diamine oxidase and zonulin. In addition, this strain significantly down-regulated the expression of inflammation-associated protein（NF-κB and TNF-α） in the liver and up-regulated the expression of ZO-1, occludin and claudin-1, associated with intestinal permeability in the ileum. Conclusion： L. plantarum C88 is a probiotic with hypoglycemic activity.