不同强度超声波对多孔钛合金支架上成骨细胞生物学行为的影响

Study of the Biological Behavior of Osteoblasts on Porous Titanium Alloy Scaffold Materials Using Different Intensities Ultrasound

目的评价不同强度超声波对经微弧氧化表面修饰的多孔钛合金(简称多孔MT)支架上成骨细胞增殖和成骨向分化的生物学行为的影响,筛选出最佳的超声强度。方法将小鼠MC3T3-E1细胞与经微弧氧化修饰的多孔MT支架材料复合培养,材料被随机分成0 MT、10 MT、30 MT、60 MT、100 MT 5组,每组分别加载不同强度的超声(0、10、30、60、100 m W/cm^2)。通过扫描电子显微镜(SEM)、噻唑蓝(MTT)值、增殖指数(PI)、碱性磷酸酶(ALP)值观察成骨细胞黏附、增殖和分化水平。结果 SEM显示各组材料表面细胞伸展良好,可见细胞长入材料孔径内部。1、3、7 d时,100 MT组MTT值低于其他组(P<0.05),而其他4组间MTT值无统计学差异。5 d时100 MT组PI值低于其他组(P<0.05)。1、3、7 d时,30 MT组ALP值明显高于其他组(P<0.05)。结论 30m W/cm2强度的超声波对多孔MT上成骨细胞增殖和成骨向分化的生物学行为影响最佳。

Objective To evaluate the biological behavior of osteoblasts on porous titanium alloy scaffold materials by using different ultrasound intensities,screening out the optimum intensity. Methods According to different ultrasound intensities（0,10,30,and 100 m W/cm2）,the materials were randomly divided into 5 groups（0,10,30,60,and 100 MT）. The MC3 T3-E1 pre-osteoblasts were seeded onto the materials in each group. The adhesion,proliferation,and differentiation rates of the osteoblasts were evaluated using scanning electron microscopy（SEM）,and methyl thiazolyldiphenyl tetrazolium bromide（MTT） assay,proliferation index（PI）,and alkaline phosphatase（ALP） assay. Results SEM imaging revealed that the cells on each group spread well and grew into the material inside the aperture. The MTT values at 1,3,and 7 d in the 100 MT group were lower than those in the other groups,with a statistically significant difference（P〈0.05）. However,no statistically significant difference was found between the other four groups. The PI value in the 100 MT group at 5 d was lower than that in the other groups（P〈0.05）,with a statistically significant difference. The ALP values at 1,3,and 7 d in the 30 MT group were significantly higher than those in the other groups,with statistically significant differences（P〈0.05）. Conclusion The optimum ultrasound intensity was 30 m W/cm-2 under this experimental condition.