急性白血病骨髓组织中PRPS1、JAB1表达量与肿瘤细胞凋亡、侵袭的相关性研究

The correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells

目的:研究急性白血病骨髓组织中PRPS1、JAB1表达量与肿瘤细胞凋亡、侵袭的相关性。方法:选择2015年2月~2017年12期间在鄂东医疗集团黄石市中心医院经骨髓穿刺活检诊断为急性白血病的患者作为研究的AL组,另取同期在鄂东医疗集团黄石市中心医院接受骨髓穿刺活检且未发现明显异常的患者作为研究的对照组。抽提RNA后测定PRPS1、JAB1以及凋亡基因、侵袭基因的mRNA表达量,抽提蛋白后测定PRPS1、JAB1的蛋白表达量。结果:AL组骨髓组织中PRPS1、JAB1的mRNA表达量及蛋白表达量均显著高于对照组;AL组骨髓组织中AuroraA、Bcl-2、β-catenin、MMP2、MMP9、N-cadherin、Msi2的mRNA表达量显著高于对照组,Bax、C/EBPα、RUNX3、TIMP3的mRNA表达量显著低于对照组,且AL组骨髓组织中PRPS1、JAB1的mRNA表达量与AuroraA、Bcl-2、β-catenin、MMP2、MMP9、N-cadherin、Msi2的mRNA表达量呈正相关,与Bax、C/EBPα、RUNX3、TIMP3的mRNA表达量呈负相关。结论:急性白血病骨髓组织中PRPS1、JAB1的高表达能够阻碍细胞凋亡、促进细胞侵袭。

Objective：To study the correlation of PRPS1 and JAB1 expression in the bone marrow tissue of acute leukemia with the apoptosis and invasion of tumor cells.Methods：Patients who were diagnosed with acute leukemia by bone marrow aspiration biopsy in Edong medical group Huangshi central hospital between February 2015 and December 2017 were selected as the AL group of the research,and patients who received bone marrow aspiration biopsy and were without significant abnormality in Edong medical group Huangshi central hospital during the same period were selected as the control group of the research.RNA was extracted to determine the mRNA expression of PRPS1,JAB1,apoptosis genes and invasion genes,and protein was extracted to determine the protein expression of PRPS1 and JAB1.Results：Both mRNA expression and protein expression of PRPS1 and JAB1 in bone marrow tissues of AL group were significantly higher than those of control group;AuroraA,Bcl-2,β-catenin,MMP2,MMP9,N-cadherin and Msi2 mRNA expression in bone marrow tissues of AL group were significantly higher than those of control group whereas Bax,C/EBPα,RUNX3 and TIMP3 mRNA expression were significantly lower than those of control group,and the PRPS1 and JAB1 mRNA expression in bone marrow tissues of AL group were positively correlated with AuroraA,Bcl-2,β-catenin,MMP2,MMP9,N-cadherin and Msi2 mRNA expression,and negatively correlated with Bax,C/EBPα,RUNX3 and TIMP3 mRNA expression.Conclusion：The high expression of PRPS1 and JAB1 in the bone marrow tissue of acute leukemia can inhibit cell apoptosis and promote cell invasion.