肺癌根治术前不同化疗方案对癌细胞生长及机体抗肿瘤免疫应答的影响

Effects of different chemotherapy regimens before radical operation for lung cancer on cancer cell growth and anti-tumor immune response

目的:探讨肺癌根治术前不同化疗方案对癌细胞生长及机体抗肿瘤免疫应答的影响。方法:选择2013年2月~2017年8月间在本院接受手术治疗的原发性肺癌患者180例,根据术前所用新辅助化疗方案不同将入组患者分为接受顺铂+紫杉醇化疗的顺铂组93例,及接受洛铂+紫杉醇化疗的洛铂组87例,对比两组患者肿瘤组织中增殖基因、凋亡基因的表达量,血清中Th1/Th2细胞因子含量的差异。结果:洛铂组肿瘤组织中增殖基因DDX17、GPx1、MACC1、RACK1、SIRT1mRNA的表达量低于顺铂组,LRRC3B mRNA的表达量高于顺铂组;凋亡基因Fas、FasL、Caspase-3 mRNA的表达量高于顺铂组,Survivin、Bcl-2mRNA的表达量低于顺铂组;血清中Th1细胞因子干扰素γ(IFN-γ)、白介素(IL)-12的含量高于顺铂组,Th2细胞因子IL-5、IL-10的含量低于顺铂组(P<0.05)。结论:肺癌根治术前洛铂化疗较顺铂化疗可更为有效的抑制肺癌细胞增殖活力并增强凋亡活力,优化机体抗肿瘤免疫应答。

Objective：To investigate the effects of different chemotherapy regimens before radical operation for lung cancer on cancer cell growth and antitumor immune response.Methods：A total of 180 patients with primary lung cancer who underwent surgery in this hospital between February 2013 and August 2017 were divided into the cisplatin group（n=93）who received cisplatin＋ paclitaxel chemotherapy and the lobaplatin group（n=87）who received lobaplatin＋ paclitaxel chemotherapy according to different preoperative neoadjuvant chemotherapy regimens.The differences in the expression of proliferation genes and apoptosis genes in tumor tissues as well as the contents of Th1/Th2 cytokines in serum were compared between the two groups.Results：Proliferation genes DDX17,GPx1,MACC1,RACK1 and SIRT1 mRNA expression levels in tumor tissue of lobaplatin group were lower than those of cisplatin group whereas LRRC3 BmRNA expression level was higher than that of cisplatin group;apoptosis gene Fas,FasL and Caspase-3 mRNA expression levels were higher than those of cisplatin group whereas Survivin and Bcl-2 mRNA expression levels were lower than those of cisplatin group;serum Th1 cytokines IFN-γand IL-12 contents were higher than those of cisplatin group whereas Th2 cytokines IL-5 and IL-10 contents were lower than those of cisplatin group（P〈0.05）.Conclusion：Lobaplatin chemotherapy before radical operation for lung cancer is more effective than cisplatin chemotherapy to inhibit the proliferation activity and enhance the apoptosis activity of lung cancer cells,and optimize the anti-tumor immune response.