金刚藤多糖对慢性盆腔炎大鼠炎症介质、细胞凋亡及免疫细胞功能的影响

Effects of smilax bockii warb polysaccharide on inflammatory mediators,apoptosis and immune cell function in rats with chronic pelvic inflammatory disease

目的:研究金刚藤多糖对慢性盆腔炎大鼠炎症介质、细胞凋亡及免疫细胞功能的影响。方法:选择雌性SD大鼠作为实验动物并分为假手术组、盆腔炎组、金刚藤组,盆腔炎组和金刚藤组建立盆腔炎模型、金刚藤组给予金刚藤胶囊灌胃。造模及干预21d后,测定子宫肌中炎症介质含量、凋亡基因表达量及外周血中免疫细胞含量。结果:盆腔炎组大鼠子宫肌组织中TLR4、NF-κB、TNF-α、ICAM-1、IL-8的含量以及Fas、TNFR-1、GRP78、CHOP、Caspase-3的mRNA表达量以及外周血中CD3^+CD8^+T细胞均高于假手术组,外周血中CD3^+CD4^+T细胞、红细胞C3b受体花环率、红细胞免疫复合物花环率低于假手术组;金刚藤组大鼠子宫肌组织中TLR4、NF-κB、TNF-α、ICAM-1、IL-8的含量以及Fas、TNFR-1、GRP78、CHOP、Caspase-3的mRNA表达量以及外周血中CD3^+CD8^+T细胞均低于盆腔炎组,外周血中CD3^+CD4^+T细胞、红细胞C3b受体花环率、红细胞免疫复合物花环率高于盆腔炎组。结论:金刚藤多糖用于慢性盆腔炎干预能够抑制炎症介质分泌及细胞凋亡、调节免疫细胞功能。

Objective：To study the effects of smilax bockii warb polysaccharide on inflammatory mediators,apoptosis and immune cell function in rats with chronic pelvic inflammatory disease.Methods：Female SD rats were chosen as experimental animals and divided into control group,pelvic inflammation group and smilax bockii warb group,pelvic inflammation group and smilax bockii warb group were made into pelvic inflammation models,and smilax bockii warb group were given intragastric administration of smilax bockii warb.The contents of inflammatory mediators and the expression of apoptosis genes in uterine muscle as well as the contents of immune cells in peripheral blood were determined 21 days after model making and intervention.Results：TLR4,NF-κB,TNF-α,ICAM-1 and IL-8 levels as well as Fas,TNFR-1,GRP78,CHOP and Caspase-3 mRNA expression in uterine muscle tissue as well as CD3~＋CD8~＋T cell in peripheral blood of pelvic inflammation group were higher than those of control group whereas peripheral blood CD3~＋CD4~＋T cell,red blood cell C3 breceptor rosette rate and erythrocyte immune complex rosette were lower than those of control group;TLR4,NF-κB,TNF-α,ICAM-1 and IL-8 levels as well as Fas,TNFR-1,GRP78,CHOP and Caspase-3 mRNA expression in uterine muscle tissue as well as CD3~＋CD8~＋T cell in peripheral blood of smilax bockii warb group were lower than those of pelvic inflammation group whereas peripheral blood CD3~＋CD4~＋T cell,red blood cell C3b receptor rosette rate and erythrocyte immune complex rosette were higher than those of pelvic inflammation group.Conclusion：Smilax bockii warb polysaccharide for chronic pelvic inflammatory disease intervention can inhibit the inflammatory mediator secretion and apoptosis and regulate the immune cell function.