病毒性心肌炎患者外周血IL-17A基因多态性与心肌损伤、MMPs/TIMPs平衡的相关性

The correlation of peripheral blood IL-17A gene polymorphism with myocardial injury and MMPs/TIMPs balance in patients with viral myocarditis

目的:研究病毒性心肌炎患者外周血IL-17A基因多态性与心肌损伤、MMPs/TIMPs平衡的相关性。方法:选择延安大学附属医院东关分院诊断为病毒性心肌炎的80例患者作为研究的VMC组,另取同期在延安大学附属医院东关分院体检的100例健康志愿者作为研究的对照组。采集外周血并测定IL-17A基因rs2275913位点的多态性,采集血清并测定心肌损伤、MMPs/TIMPs指标的含量。结果:VMC组患者中IL-17A基因GG基因型所占比例低于对照组,GA+AA基因型所占比例高于对照组;VMC组患者血清中IL-17、CK-MB、cTnI、sFas、MDA、PINP、ICTP、MMP2、MMP9、TIMP1、TIMP2的含量显著高于对照组且VMC组中GA+AA基因型患者血清中IL-17、CK-MB、cTnI、sFas、MDA、PINP、ICTP、MMP2、MMP9、TIMP1、TIMP2的含量高于GG基因型患者。结论:病毒性心肌炎患者外周血IL-17A基因rs2275913位点等位基因G向A的突变能够加重心肌损伤及MMPs/TIMPs失衡。

Objective：To study the correlation of peripheral blood IL-17A gene polymorphism with myocardial injury and MMPs/TIMPs balance in patients with viral myocarditis.Methods：80 patients who were diagnosed with viral myocarditis in Dongguan Branch,Yan＇an University Affiliated Hospital between September 2014 and September 2017 were selected as the VMC group of the research,and 100 healthy volunteers who received physical examination in Dongguan Branch,Yan＇an University Affiliated Hospital during the same period were selected as the control group of the research.The peripheral blood was collected to determine the IL-17A gene rs2275913 locus polymorphism,and serum was collected to determine the contents of myocardial injury and MMPs/TIMPs indexes.Results：The proportion of IL-17A gene GG genotype of VMC group was lower than that of control group and the proportion of GA＋AA genotype was higher than that of control group;serum IL-17,CKMB,cTnI,sFas,MDA,PINP,ICTP,MMP2,MMP9,TIMP1 and TIMP2 contents of VMC group were significantly higher than those of control group,and serum IL-17,CK-MB,cTnI,sFas,MDA,PINP,ICTP,MMP2,MMP9,TIMP1 and TIMP2 contents of VMC group of patients with GA＋AA genotype were higher than those of patients with GG genotype.Conclusion：The mutation of the allele G to A in the peripheral blood IL-17A gene rs2275913 locus of patients with viral myocarditis can aggravate myocardial injury and MMPs/TIMPs imbalance.