早期结肠癌组织的干细胞特性分析及其与细胞增殖、侵袭的关系

The stem cell characteristics of early colon cancer tissue and their relationship with cell proliferation and invasion

目的:研究早期结肠癌组织的干细胞特性分析及其与细胞增殖、侵袭的关系。方法:选择东莞市人民医院2010年1月~2017年10月期间手术切除的结肠癌组织和癌旁组织作为本次研究的临床样本,抽提蛋白后测定肿瘤干细胞基因USP22、Nanog、Lgr5、CD44的蛋白表达量,抽提RNA后测定细胞增殖基因、细胞侵袭基因的mRNA表达量。结果:结肠癌组织中USP22、Nanog、Lgr5、CD44的蛋白表达量以及Rab5A、TBX2、MDM2、TGF-β1、Smad2/3、Vimentin、Rac1、VEGF的mRNA表达量均显著高于癌旁组织,Bad、Bax、Fas的mRNA表达量均显著低于癌旁组织;结肠癌组织中USP22、Nanog、Lgr5、CD44的蛋白表达量与Rab5A、TBX2、MDM2、TGF-β1、Smad2/3、Vimentin、Rac1、VEGF的mRNA表达量呈正相关,与Bad、Bax、Fas的mRNA表达量呈负相关。结论:早期结肠癌组织内干细胞特性的激活能够促进癌细胞的增殖、侵袭。

Objective：To study the stem cell characteristics of early colon cancer tissue and their relationship with cell proliferation and invasion.Methods：Colon cancer tissues and adjacent tissues surgically removed in Dongguan People＇s Hospital between January 2010 and October 2017 were selected as the clinical samples of this study,the protein was extracted to determine the protein expression of tumor stem cell genes USP22,Nanog,Lgr5 and CD44,and RNA was extracted to determine the mRNA expression of cell proliferation genes and cell invasion genes.Results：USP22,Nanog,Lgr5 and CD44 protein expression as well as Rab5A,TBX2,MDM2,TGF-β1,Smad2/3,Vimentin,Rac1 and VEGF mRNA expression in colon cancer tissues were significantly higher than those in adjacent tissues while Bad,Bax and Fas mRNA expression were significantly lower than those in adjacent tissues;USP22,Nanog,Lgr5 and CD44 protein expression in colon cancer tissues were positively correlated with Rab5A,TBX2,MDM2,TGF-β1,Smad2/3,Vimentin,Rac1 and VEGF mRNA expression,and negatively correlated with Bad,Bax and Fas mRNA expression.Conclusion：The activation of stem cell characteristics in early colon cancer can promote the proliferation and invasion of cancer cells.