27个Y-STR基因座在甘肃东乡族人群的遗传多态性

Genetic Polymorphisms of 27 Y-STR Loci in Dongxiang Population of Gansu Province

目的调查27个Y-STR基因座在甘肃东乡族人群的遗传多态性,探讨其群体遗传学关系及法医学应用价值。方法应用STRtyper-27Y试剂盒检测526名甘肃东乡族无关男性个体在27个Y-STR基因座的基因分型,计算等位基因频率及单倍型多样性。同时,结合目前国内外已公开发表的其他14个群体相同基因座的遗传学资料,分析甘肃东乡族群体的遗传距离和聚类关系。结果双等位基因座DYS385a/b检出55种单倍型,DYF387S1基因座检出39种单倍型,其余23个单拷贝STR基因座分别检出4~16个等位基因,基因多样性(gene diversity,GD)值在0.453 9(DYS391)~0.957 5(DYS385a/b)。27个Y-STR基因座在526名个体中共观察到471种单倍型,单倍型多样性为0.999 5。从遗传距离分析发现,甘肃东乡族与甘肃藏族之间的遗传距离最近(0.068 2),与河南汉族(0.084 7)之间的遗传距离相对较远,基于遗传距离所构建的多维尺度分析与聚类分析结果基本相符。结论该27个Y-STR基因座在甘肃东乡族人群中具有丰富的遗传多态性,对Y染色体数据库建立、群体遗传学研究和法医学应用有重要意义。

Objective To investigate the genetic polymorphisms of 27 Y-STR in Dongxiang population of Gansu province,and to explore the population genetic relationship and the value of forensic application.Methods The genotyping of 27 Y-STR loci in 526 unrelated male individuals in Dongxiang population of Gansu province were detected by STRtyper-27 Y kit.The allele frequencies and haplotype diversity were also calculated.Combining with other genetics data of 14 loci in same populations,which have been published at home and abroad,the genetic distance and clustering relationship in Dongxiang population of Gansu province were calculated.Results Totally 55 haplotypes were found in the DYS385 a/b biallelic loci,39 haplotypes in DYF387 S1 loci,and 4-16 alleles in the rest 23 single copy STR loci.The GD value was from 0.453 9（DYS391） to 0.957 5（DYS385 a/b）.Totally 471 haplotypes were observed in 27 Y-STR loci in 526 individuals,and the value of haplotypes diversity was 0.999 5.The genetic distance between Dongxiang and Tibetan populations of Gansu province was the closest（0.068 2）,while it was the longest between Dongxiang population in Gansu province and Han population in Henan province（0.084 7）.The result of dimensional analysis established upon the genetic distance was basically matched with that of the cluster analysis.Conclusion The 27 Y-STR loci show a high genetic polymorphism in Dongxiang population of Gansu province,which has significance for the Y-STR database establishment,population genetics study and forensic practice.