苦楝组织培养与快速繁殖技术研究

Research Progress on Tissue Culture and Rapid Propagation of Melia azedarach

以新生顶芽茎段为试验材料,筛选苦楝组织培养的最佳培养基配方,建立苦楝快繁技术体系。结果表明:最适启动培养基为WPM+0.25mg/L NAA+0.5mg/L TDZ;增殖培养基为WPM+1.5mg/L NAA+1.5mg/L 6-BA;生根培养基为1/2WPM+0.5mg/L IBA+0.1mg/L NAA+0.5g/L AC(活性炭):炼苗移栽基质以腐质土和蛭石混合,2∶1效果最好,成活率达95%。

With stem segment of newborn terminal were used experimental materials,selection of the best culture medium for the tissue culture,we will establish a tissue culture and rapid propagation system of Melia azedarach.The results are as following：The optimum medium for bud induction was WPM＋0.25 mg/L NAA＋0.5 mg/L TDZ;The optimum combination of multiplication culture was WPM＋1.5 mg/L NAA＋1.5 mg/L 6-BA;1/2 WPM＋0.5 mg/L IBA＋0.1 mg/L NAA＋0.5 g/L AC is available to culture rooting. Using the transplanting matrix was humus soil：vermiculite=2∶1,the survival rate was maximal for 95% with the ideal of seedling also was the best.