摘要
采用乙醇回流提取法,对提取温度、乙醇浓度、时间、料液比进行单因素考察,结合正交试验,确定锈毛莓总黄酮最佳提取工艺。结果显示,最佳提取工艺为料液比1∶25、乙醇浓度60%、提取温度70℃、提取时间2.5 h,提取2次,总黄酮得率可达6.78%。
Extraction and purification technology of total flavonoids in Rubus reflexus were investigated by single factor study and orthogonal experiment. Results showed the optimal conditions were material to liquid ratio with 1：25, 60% ethanol concentration, 70 ℃, extracting 2.5 hours and extraction times with twice. Through the best extraction technology, the yield of R. reflexus total flavonoids could be up to 6.78%.
作者
颜沛沛
叶齐
莫智龙
王小华
叶炜
雷伏贵
魏道智
YAN Pei-pei1, YE Qi2, MO Zhi-long1, WANG Xiao-hua2, YE Wei1, LEI Fu-gui1, WEI Dao-zhi2(1.Sanming Academy of Agricultural Sciences, Sanming 365000, Fujian China; 2.Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian China)
出处
《亚热带植物科学》
2018年第2期113-116,共4页
Subtropical Plant Science
基金
福建省自然科学基金(13131153)
关键词
锈毛莓
提取工艺
黄酮
Rubus reflexus
extraction technology
flavonoids
