摘要
木本植物因其生命周期长、基因组杂合度高、基因组较大、遗传背景不清晰等特性,制约了其研究进程。随着现代生物技术的发展,DNA分子标记技术在木本植物研究领域的应用越来越多,其中单核苷酸多态性(SNP)作为第三代分子标记技术以其高效、快速、稳定、可靠等诸多优点得到广泛应用。本文简述SNP标记的特点、开发方法、检测方法及其在木本植物遗传多样性和亲缘关系分析、品种鉴定、连锁图谱构建和辅助育种等方面的研究进展,为更好地应用SNP技术开展木本植物研究提供参考。
Research progress of ligneous plants was restricted for their long life cycle, high heterozygosity, large genome and unclear genetic background. With the development of modern biotechnology, DNA molecular markers were applied in research of ligneous plants. Single nucleotide polymorphism（SNP）, the third generation of molecular markers, has many advantages, such as high efficiency, rapidity, stability, reliability and so on, so it has been widely used. In order to provide a reference for better application of SNP technology in ligneous plant research, SNP markers characteristics, development methods, detection methods and its applications in genetic diversity and genetic relationship analysis, cultivar identification, genetic linkage map construction and molecular assisted breeding for ligneous plants were summarized.
作者
周琳
段玉
文博
马媛春
朱旭君
王玉花
房婉萍
ZHOU Lin1,2,DUAN Yu1,WEN Bo1,MA Yuan-chun1,ZHU Xu-jun1,WANG Yu-hua1,FANG Wan-ping1(1.Tea Research Institute,Nanjing Agricultural University,Nanjing 210095,Jiangshu China; 2.Forestry and Pomology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China)
出处
《亚热带植物科学》
2018年第2期187-193,共7页
Subtropical Plant Science
基金
中央高校基本科研业务费资助项目(KYZ201841
KYZ201842)
国家茶叶产业技术体系(CARS-19)
江苏省现代茶叶技术体系(SXGC[2017]279)
江苏省农业科技自主创新资金项目(CX[17]2018)
关键词
木本植物
SNP标记
遗传多样性分析
品种鉴定
指纹图谱构建
辅助育种
ligneous plant
SNP marker
genetic diversity analysis
cultivar identification
fingerprinting construction
assisted breeding
