跑步运动结合摄取杜仲提取物对去卵巢大鼠骨密度和抗氧化能力的影响

Effects of the combination of running and Eucommia ulmoides extract on the bone mineral density and antioxidant capacity in ovariectomized rats

背景:研究显示运动导致骨密度增加,并可改善身体组成。但妇女停经后身体抗氧化能力大幅下降,所以预防运动造成的氧化伤害亦非常关键。杜仲具有对改善骨密度和抗氧化效果,利用运动搭配杜仲摄入,尝试既可以改善更年期骨密度又能减少运动氧化伤害的方案。目的:探讨跑步运动结合摄取杜仲提取物和单纯摄入杜仲提取物对接受卵巢切除大鼠骨密度、血清雌激素、骨质代谢、抗氧化能力的影响。方法:先对大鼠进行1周适应性饲养,随机将大鼠分为2组,分别进行卵巢切除或假手术。大鼠恢复4周后,将切除卵巢大鼠随机分为模型组、杜仲组,杜仲运动组,进行12周干预,干预结束后,将所有大鼠置入代谢笼采集24 h尿样;隔日,所有大鼠进行力竭运动,之后采集大鼠腹腔血液。检测各组大鼠的氧化指标(包括血肌酸激酶、乳酸脱氢酶浓度、丙二醛、超氧化物歧化酶、谷胱甘肽过氧化酶活性),骨质代谢指标(包括血清骨钙素、碱性磷酸酶、Ca、P浓度以及尿液中的脱氧吡啶啉、Ca、P水平),同时记录大鼠的体质量变化、血清雌二醇水平和股骨骨密度值。结果与结论:经12周干预后,杜仲组和杜仲运动组大鼠体质量、丙二醛、骨钙、碱性磷酸酶、磷浓度显著低于模型组(P<0.05);血清雌二醇、股骨骨密度显著高于模型组(P<0.05)。杜仲运动组各项指标均优于杜仲组和运动组,但未达到显著性差异(P>0.05)。说明长期进行跑步运动并结合摄取杜仲提取物或单纯摄取杜仲提取物均可以显著提升切除卵巢骨质疏松大鼠的骨密度、血清雌激素水平,并可以促进骨代谢平衡,降低骨转换率和因卵巢功能降低导致的体质量增加,能够起到维护骨量、防止骨质疏松作用。

BACKGROUND： Exercise has been shown to increase bone mineral density（BMD） and improve body composition. Nevertheless, anti-oxidant capacity in postmenopausal women is significantly reduced, and it is crucial to prevent exercise-induced oxidative damage. Eucommia ulmoides has the abilities of increasing BMD and antioxidant capacity, so the combination of exercise and Eucommia ulmoides may be helpful to increase BMD and reduce oxidative damage in postmenopausal women. OBJECTIVE： To investigate the effects of the combination of running and Eucommia ulmoides extract on the BMD, serum level of estrogen, bone metabolism and antioxidant capacity in ovariectomized rats. METHODS： After the rats were allowed to acclimate for 1 week, the rats were randomly divided into two groups： ovariectomized and sham operation groups. The rats were recovered for 4 weeks, and the ovariectomized rats were randomly divided into model, Eucommia ulmoides and combination（Eucommia ulmoides and running） groups. The intervention time was 12 weeks. The 24-hour urine was recorded. All rats underwent exhaustive exercise on the following day and the blood samples from the abdominal cavity were collected. The oxidative indexes（serum levels of creatine kinase, lactate dehydrogenase, malondialdehyde, and superoxide dismutase, and glutathione peroxidase activity）, bone metabolism indexes（serum levels of osteocalcin, alkaline phosphatase, creatine kinase, calcium and phosphorus, and urine levels of deoxypyridinoline, calcium and phosphorus）, body mass, serum level of estradiol and femoral BMD were recorded. RESULTS AND CONCLUSION： After 12 weeks of intervention, the body mass, levels of malondialdehyde, calcium, alkaline phosphatase and phosphorus in the Eucommia ulmoides and combination groups were significantly lower than those in the model group（P〈0.05）. The serum level of estradiol and femoral BMD were significantly higher than those in the model group（P〈0.05）. The indexes in the combination group all were superior to the Eucommia ulmoides and exercise groups（P〉0.05）. These findings suggest that long-term running combined with Eucommia ulmoides extract or simple Eucommia ulmoides extract can significantly increase BMD, serum level of estrogen in ovariectomized rats, and can promote bone metabolism balance, reduce bone turnover rate and weight gain caused by ovarian dysfunction, thus maintaining bone mass and preventing osteoporosis.