miR-7在口腔鳞状细胞癌中的表达及潜在临床意义

Expression and potential clinical significance of miR-7 in oral squamous cell carcinoma

目的研究miR-7在口腔鳞状细胞癌(OSCC)中的表达及潜在临床意义。方法获取原发性OSCC患者肿瘤组织53例及正常健康组织27例,利用qRT-PCR检测各组织中miR-7的表达,并分析其与上皮间质转化的相关性。利用质粒转染技术将含miR-7序列片段的质粒导入OSCC细胞株SCC9中,通过建立过表达miR-7的SCC9细胞进一步验证miR-7与OSCC上皮间质转化的关系。结果与健康组织比较,miR-7在OSCC肿瘤组织中的表达显著降低(P<0.01);miR-7的表达与上皮间质转化过程呈显著相关性(P<0.001)。与对照组细胞比较,过表达miR-7促进SCC9细胞E-cadherin的表达,抑制Fibronectin的表达(P<0.01)。结论miR-7在OSCC中低表达,与上皮间质转化过程高度相关。

Objective To investigate the expression and potential clinical significance of miR-7 in oral squamous cell carcinoma（OSCC）.Methods Cancer tissues from 53 OSCC patients and healthy tissue from 27 people were collected.The levels of miR-7 in each tissue were detected by quantitative RT-PCR;the correlation between miR-7 and epithelial mesenchymal transition（EMT） were analyzed.Plasmid of miR-7 was conducted into SCC9 cells to confirm the role of miR-7 in OSCC.Results Compared with the healthy tissue,the levels of miR-7 in OSCC tissue were decreased significantly（P0.01）;the expression of miR-7 in OSCC tissue were highly correlated with epithelial mesenchymal transition（P0.001）.Compared with the control cells,overexpression of miR-7 promoted E-cadherin expression,but inhibited Fibronectin expression in SCC9 cells（P0.01）.Conclusion miR-7 is low expressed in OSCC,which is highly correlated with epithelial-mesenchymal transition.