人参皂苷Re对防龋疫苗rPAc免疫效应的影响

The effect of Re on immune response against subunit anti-caries vaccie rPAc

目的研究人参皂苷Re作为防龋亚单位疫苗rPAc佐剂的免疫保护效应。方法 40只BALB/c小鼠随机分为4组:防龋疫苗(rPAc)组,人参皂苷Re(Re)组,Re+rPAc组和生理盐水(NS)组,分别用rPAc、Re、Re+rPAc、生理盐水经鼻黏膜免疫。2周后加强免疫1次。用酶联免疫吸附试验(ELISA)检测血清和唾液中特异性抗体的水平。脾细胞刺激实验检测细胞因子分泌水平。将24只定植了变形链球菌的Wistar大鼠随机分为4组:rPAc组,Re组,Re+rPAc组和NS组,分别用rPAc、Re、Re+rPAc、生理盐水经鼻黏膜免疫。2周后加强免疫1次。龋齿记分评价防龋保护效能。结果 Re+rPAc组血清特异性抗PAc IgG、IgG1、IgG2a水平及唾液中特异性抗PAc IgA抗体水平均明显高于其他组(P<0.01)。Re+rPAc组较其他组脾淋巴细胞白细胞介素4和γ干扰素表达增强(P<0.05)。大鼠龋齿保护实验显示Re+rPAc组定菌鼠釉质龋(E)、牙本质浅龋(Ds)和牙本质中龋(Dm)均显著低于其他组(P<0.05)。结论 Re作为防龋亚单位疫苗rPAc的佐剂可提高防龋疫苗的血清和唾液中特异性抗体的水平及防龋保护效能,具有作为防龋疫苗rPAc免疫佐剂的可行性。

Objective To study the immune efficacy of ginsenoside Re as the adjuvant of anti-caries subunit vaccine rPAc.Methods 40 mice were randomly diveded into four groups：anti-caries vaccine group（rPAc group）,ginsenoside Re groups（Re group）,Re ＋ rPAc group,normal saline group（NS group）.Mice were intranasally immunized twice on weeks 0 and 2 with rPAc,Re,Re ＋ rPAc or normal saline,respectively.Concentration of specific antibodies in serum and saliva were detected by enzyme-linked immunosorbent assay（ELISA）.In addition,cytokine production of spleen lymphocyte responses were also evaluated.24 Wistar rats,infected with S.mutans Ingbritt,were randomly diveded into four groups：rPAc group,Re group,Re ＋ rPAc group,NS group.Rats were jmmunized intranasally with rPAc,Re,Re ＋ rPAc or normal saline,respectively.The Keyes method was used to determine the caries activity.Results The level of serum specific anti-PAc IgG,IgG1,IgG2 a and saliva anti-PAc IgA were significantly higher in Re ＋ rPAc group（P0.05）.In addition,the expression of cytokines interluekin-4 and interferon-γ were significantly upregulated in Re ＋ rPAc group than those in any other groups（P0.01）.Re ＋ rPAc immunized rats showed significantly fewer E,Ds and Dm lesions than rPAc-immunized rats,Re-immunized rats or NSimmunized rats（P0.05）.Conclusion Re as the adjuvant of anti-caries subunit vaccine rPAc triggers a stronger humoral and cellular response against dental caries,and Re is a promising adjuvant for anti-caries vaccine rPAc.