他克莫司和伊曲康唑联合使用体外抗克柔念珠菌作用的研究

Study on the anti-Candida krusei in vitro combined with tacrolimus and itraconazole

目的单独应用抗真菌剂伊曲康唑和钙调神经磷酸酶抑制剂他克莫司,以及上述两种药物联合使用后,评估体外实验对克柔念珠菌生长的影响情况。是否联合用药比单独应用抗真菌剂更有效抑制克柔念珠菌的生长。方法根据美国临床及实验室标准协会指南的方法,将12株克柔念珠菌通过微量稀释法分为空白对照组、伊曲康唑组、他克莫司组以及联合用药组,然后将12株克柔念珠菌孵育24 h后,观察其生长情况。结果与单独使用伊曲康唑药物相比,联合用药作用下伊曲康唑最低抑菌浓度远小于单独使用伊曲康唑,其最高倍数达32倍,最小倍数达4倍;同样,他克莫司单独使用较联合伊曲康唑使用,最高倍数达32倍,最低倍数达2倍。结论 12株克柔念珠菌在伊曲康唑和他克莫司联合使用下,体外实验表现出强大的协同作用。

Objective The use of the antifungal agent itraconazole and the calcineurin inhibitor tacrolimus alone,together with the above two drugs,was evaluated in vitro for growth of Candida krusei.Whether or not the combination is more effective than the antifungal alone can inhibit the growth of Candida krusei.Methods According to the guidelines of the American Institute of Clinical and Laboratory Standards,12 Candida krusei strains were divided into blank control group,itraconazole group,tacrolimus group,and combination group by microdilution method,and then 12 the Candida krusei strain was incubated for 24 h and its growth was observed.Results In comparison with the alone use of isotriconazole in Candida krusei,the combined use of itraconazole,its minimal inhibitory concentration was much less than that of alone,with a maximum multiple of 32 fold,a minimum multiple up to 4 times;the same,In comparison with the alone use of tacrolimus in Candida krusei,the combined use of tacrolimus,its maximum multiple of 32 times,the lowest multiple of 2 times.Conclusion The combined use of itraconazole and tacrolimus in 12 strains of Candida krusei displayed a powerful synergistic effect in vitro.