摘要
建立高效液相色谱法(high performance liquid chromatography,HPLC)快速测定藻油中二十二碳六烯酸乙酯(docosahexaenoic acid ethyl ester,DHA-EE)含量的方法。将藻油经乙酯化处理后,采用优化后DHA-EE分析参数:色谱柱ZORBAX SB-C8(4.6 mm×250 mm),流动相V(甲醇)∶V(水)=90∶10,柱温为30℃,流速为1.0 m L/min,紫外检测波长为204 nm,进样量为10μL。测定结果显示,DHA-EE在0.008 1~1.92μg范围内呈良好线性关系,线性回归方程为:y=39.91 x+765.11(R2=0.999 2),平均回收率为102.42%(n=5,RSD=2.25%),精密度(n=10,RSD=0.32%)较好,DHA-EE最低定量限达8.05 ng。表明,该方法可用于藻油及其制品中DHA-EE的检测分析。
High performance liquid chromatography（ HPLC） method was developed to quantify DHAEE in marine microalgae oil quickly. The optimized quality parameters for DHA-EE analysis were as follows：chromatographic column,ZORBAX SB-C8（ 4. 6 mm × 250 mm）; mobile phase,methanol versus water（90∶ 10）; the column temperature 30 ℃; the flow velocity 1. 0 m L/min; UV wavelength 204 nm; the injection volume 10 μL. It presented a good linear relationship within range of 0. 008 1-1. 92 μg for DHA-EE,and the linear regression equation was y = 39. 91 x ＋ 765. 11（ R2= 0. 999 2）. The average recovery rate was102. 42%（ n = 5,RSD = 2. 25%） with good precision（ n = 10,RSD = 0. 32%）,and the minimum quantify limit reached 8. 05 ng. This optimized HPLC method could be used in the determination and analysis of algal oil and DHA-EE.
作者
谢枫才
张宾
孙继鹏
XIE Fengcai1,2, ZHANG Bin1, SUN Jipeng2(1. College of Food Science and Phamlacy, Zhejiang Ocean University, Zhoushan 316022, China; 2. Third Institute of Oceanography, State Oceanic Administration, Engineering Research Center of Marine Biological Resource Comprehensive Utilization, Xiamen 361005, China)
出处
《集美大学学报（自然科学版）》
CAS
2018年第3期187-194,共8页
Journal of Jimei University：Natural Science
基金
国家重点研发计划项目(2017YFD0400105-05)
厦门市海洋经济创新发展区域示范项目(16CZP012SF04)
