明胶/掺锶β-磷酸三钙/硫酸钙复合多孔支架的制备与性能

Fabrication and Properties of Porous Gelatin/Strontium-doped β-tricalcium Phosphate/Calcium Sulfate Scaffolds

以掺锶β-磷酸三钙/硫酸钙为原料,利用搅拌喷雾干燥法制备出掺锶β-磷酸三钙/硫酸钙复合小球,再将明胶与制备的复合小球复合,制备出明胶/掺锶β-磷酸三钙/硫酸钙复合生物支架。通过X射线衍射仪、扫描电镜、红外光谱仪等分析了复合多孔支架的成分、形貌以及结构特征,并研究了复合生物支架的降解性、孔隙率、力学性能和细胞毒性等。结果表明:该复合多孔生物支架具有不规则的孔洞结构,孔径在0.5~1mm之间,且平均孔隙率达到(63±1.77)%,基本能满足骨组织工程支架对孔隙率的要求;复合多孔支架具有良好的抗压强度,其平均抗压强度在(6.3±0.05)MPa左右;该复合多孔支架无细胞毒性,具有较好的降解速率,因此该支架在骨组织修复方面具有良好的应用前景。

The present work fabricated the calcium sulfate/strontium-doped β-tricalcium phosphate composite spherical pellets through the stirring spray drying method and using the calcium sulfate/strontium-dopedβ-TCP as raw material.Subsequently the composite spherical pellets were combined with gelatin,to get porous gelatin/calcium sulfate/strontium-dopedβ-tricalcium phosphate scaffolds.We employed X-ray diffraction（XRD）,scanning electron microscopy（SEM）and Fourier transform infrared spectroscopy（FT-IR）,etc.to examine the products＇ chemical composition,morphology and microstructure,and also investigated the degradability,porosity,mechanical properties and cytotoxicity.The results reveal that the composite porous scaffolds have an irregular pore structure with pore size ranging from 0.5 mm to 1.0 mm and the porosity of about（63±1.77）%,which could meet the requirements of scaffold for bone tissue engineering.Besides,the favorable compressive strength（average value（6.3±0.05）MPa）of the resultant scaffolds were observed.The cytotoxicity test showed that,the composite scaffolds exhibit no cytotoxic effect and good degradation rate and thereby can find potential application in bone tissue repair.