地五养肝胶囊对二乙基亚硝胺诱导斑马鱼肝损伤的保护作用

Protective effects of Diwu Yanggan Capsules on diethylnitrosamine-induced liver injury in zebrafishes

目的考察地五养肝胶囊对二乙基亚硝胺诱导斑马鱼肝损伤的保护作用。方法 360条斑马鱼随机分成空白组(标准培养液)、模型组(二乙基亚硝胺)、地五养肝胶囊干预组(25、50、100μg/m L)、异甘草酸镁注射液干预组(0.1 mg/m L)。7 d后,测量斑马鱼肝脏面积,计算其相对光密度,整体油红O染色法测定肝内脂滴数。结果模型组肝脏浑浊,其面积较空白组显著减小(P<0.001),相对光密度显著升高(P<0.05),脂滴密集分布;50μg/m L地五养肝胶囊干预组及异甘草酸镁注射液干预组肝脏更清晰,脂滴更少,肝脏面积、相对光密度与空白组比较均无明显差异(P>0.05)。结论地五养肝胶囊对二乙基亚硝胺致斑马鱼肝损伤具有一定保护作用。

AIM To investigate the protective effects of Diwu Yanggan Capsules on diethylnitrosamine-induced liver injury in zebrafishes. METHODS Three hundred and sixty zebrafishes were randomly divided into blank group（ E3 solution）,model group（ diethylnitrosamine）,Diwu Yanggan Capsules intervention groups（ 25,50,100 μg/m L） and Magnesium Isoglycyrrhizinate Injection intervention group（0. 1 mg/m L）. After 7 d,the areas of zebrafish livers were measured,whose relative optical densities were calculated,and whole oil red O staining was applied to determining the amount of lipid droplets in liver. RESULTS The turbid livers in the model group demonstrated significantly smaller areas（ P 〈 0. 001） and higher relative optical densities（ P 〈 0. 05） than those in the blank group,with the dense distribution of lipid droplets. The groups intervened with 50 μg/m L Diwu Yanggan Capsules and Magnesium Isoglycyrrhizinate Injection had clearer livers and fewer lipid droplets. No significant differences in liver areas and relative optical densities were observed in these two groups in contrast to those in the blank group（ P 〉 0. 05）. CONCLUSION Diwu Yanggan Capsules have certain protective effects on diethylnitrosamine-induced liver injury in zebrafishes.