UPLC法同时测定狼毒大戟中岩大戟内酯A和B

Simultaneous determination of jolkinolides A and B in Euphorbia fischeriana by UPLC

目的建立UPLC法同时测定狼毒大戟Euphorbia fischeriana Steud.中岩大戟内酯A和B的含有量。方法狼毒大戟甲醇提取物的分析采用Waters BEH C_(18)色谱柱(2.1 mm×50 mm,1.7μm);流动相乙腈-水,梯度洗脱;体积流量0.4 mL/min;检测波长250 nm;柱温35℃。结果岩大戟内酯A和B分别在1.804~54.12μg/mL(r=0.999 5)和9.663~209.8μg/mL(r=0.999 8)范围内线性关系良好,平均加样回收率分别为98.3%、98.6%,RSD分别为0.76%、0.77%。结论该方简便准确,重复性好,可用于狼毒大戟的质量控制。

AIM To establish a UPLC method for the simultaneous content determination of jolkinolide A and B in Euphorbia fischeriana Steud.. METHODS The analysis of methanol extract of E. fischeriana was performed on a 35 ℃ Waters BEH C18 column（2. 1 mm × 50 mm,1. 7 μm）,with the mobile phase comprising of acetonitrile-water flowing at 0. 4 mL/min in a gradient elution manner,and the detection wavelength was set at 250 nm.RESULTS Jolkinolide A and B showed good linear relationships within the ranges of 1. 804-54. 12 μg/mL（r = 0. 999 5） and 9. 663-209. 8 μg/mL（r = 0. 999 8）,whose average recoveries were 98. 3%,98. 6% with the RSDs of 0. 76%,0. 77%,respectively. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of E. fischeriana.