龙眼肉不同提取物的神经保护作用及其机制

Neuroprotective Effects of different Parts of Longan Meat and its Mechanism

目的:探讨龙眼肉的神经保护作用及其机制。方法:采用Aβ诱导PC12细胞损伤,用龙眼肉不同提取物作用于损伤的PC12细胞,Elisa试剂盒检测SOD、MDA水平,MTT检测细胞增殖;采用H2O2诱导PC12细胞损伤,Annexin V-FITC/PI双染色法检测细胞凋亡。结果:龙眼肉水提取物和正丁醇提取物能提高Aβ诱导损伤的细胞存活和SOD活力,抑制H2O2诱导损伤的早期细胞凋亡,对MDA含量无明显影响;乙酸乙酯提取物可减少H2O2诱导损伤的各期细胞凋亡及坏死,但对Aβ诱导损伤的细胞存活以及SOD活力和MDA含量无明显影响。结论:龙眼肉具有神经细胞保护作用,但不同提取物作用机制可能不同。

Objective To explore the neuroprotective effect and mechanism of longan meat. Methods Using Aβ to induce PC12 cell injury,with different extracts of longan meat on the injured PC12 cells,Elisa kit to detect SOD、MDA levels,MTT assay for cell proliferation; H_2O_2 induced PC12 cell injury,detection of Apoptosis by Annexin V-FITC/PI Double Staining. Results Longan meat extract and n-butanol extract can improve the cell viability and SOD activity,inhibition of early apoptosis,and no significant effect on MDA content induced by Aβ; Ethyl acetate extract can reduce cell apoptosis and necrosis induced by H_2O_2,but no significant effect on cell viability,SOD activity and MDA content induced by Aβ. Conclusion Longan meat extract has a neuroprotective effect,but the mechanisms of different extraction may be different.