探讨BMSCs对COPD模型大鼠中炎症因子及血气的影响

To Investigate the Effect of BMSCs on Inflammatory Factors and Blood Gas in COPD Rats

目的观察骨髓间充质干细胞(BMSCs)对慢性阻塞性肺疾病(COPD)模型大鼠中炎症因子及血气分析的影响。方法选取24只健康雄性大鼠,按照1:1:1比例,将其分为A组(正常组,n=8)、B组(模型组,n=8)和C组(治疗组,n=8)。其中,A组不做任何处理,B组、C组均建立COPD模型。三组大鼠均以普通饲料持续喂养76天。C组在尾静脉注射BMSCs,其他两组则注射等量的生理盐水,继续喂养2周后处死大鼠。观察三组炎症因子、血气分析。结果 B组IL-1β、TNF-α高于A组、C组,且C组高于A组(P<0.05);三组p H、Sa O2、PCO2、PO2水平两两对比,差异均无统计学意义(P>0.05)。结论骨髓间充质干细胞可影响慢性阻塞性肺疾病的血气改变,且能抑制炎症反应,需引起高度关注。

Objective To observe the effects of bone marrow mesenchymal stem cells（BMSCs） on inflammatory factors and blood gas analysis in chronic obstructive pulmonary disease（COPD） rats. Methods 24 healthy male rats were divided into group A（normal group, n =8）, group B（model group, n =8） and group C（treatment group, n =8） according to the proportion of 1： 1： 1. Among them, A group did not do any treatment, B group and C group all set up COPD model. The three groups of rats were fed with regular feed for 76 days. In group C, BMSCs was injected into the caudal vein. The other two groups were injected with the same amount of normal saline. After 2 weeks of feeding, rats were sacrificed. The three groups of inflammatory factors and blood gas analysis were observed. Results IL-1βand TNF-α in group B were higher than that of group A and C, and group C was higher than group A（P〈0.05）, and there was no significant difference between the three groups of p H, Sa O2, PCO2, and PO2. Conclusion Bone marrow mesenchymal stem cells can affect the blood gas changes of chronic obstructive pulmonary disease, and can inhibit theinflammatory response.