胃癌患者外周血中CD4^+CD25^+调节性T细胞和Th17细胞的表达和临床意义

The expression and clinical significance of CD4^+CD25^+regulatory T cells and Th17 cells in peripheral blood of patients with gastric carcinoma

目的检测CD4^+CD25^+调节性T细胞和Th17细胞在胃癌患者外周血中的表达,探讨两种细胞在胃癌中的临床意义。方法随机选取我院收治的胃癌患者30例,作为胃癌组,另选取同期在我院进行健康体检人员30例作为正常组,对两组外周血中CD4、CD25、Foxp3、IL-17的表达进行检测,分析CD4^+CD25^+调节性T细胞和Th17细胞的数量和比例。结果胃癌组外周血中CD4^+CD25^+调节性T细胞和Th17细胞的表达均高于正常组(P<0.05);胃癌组中晚期、T3+T4、有淋巴结转移患者外周血中CD4^+CD25^+调节性T细胞的表达分别高于早期、T1+T2、无淋巴结转移患者(P<0.05),但不同肿瘤分期、浸润深度、淋巴结转移患者之间Th17细胞的表达差异均不显著(P>0.05)。结论胃癌患者外周血中CD4^+CD25调节性T细胞和Th17细胞的表达均显著升高,且提示肿瘤进展和CD4^+CD25^+调节性T细胞相关,但与Th17细胞无明显统计学相关。

Objective To assess the expression and discuss the clinical significance of CD4＋CD25＋regulatory T cells and Th17 cells in peripheral blood of patients with gastric carcinoma. Methods 30 patients with gastric carcinoma in our hospital were randomly selected as the gastric carcinoma group. 30 people who checked up their health condition in our hospital during the same period were selected as the normal group. The expression of CD4, CD25, Foxp3 and IL-17 in peripheral blood was assessed in both groups. The amount and proportion of CD4＋CD25＋regulatory T cells and Th17 cells were analyzed. Results The expression of CD4＋CD25＋regulatory T cells and Th17 cells in gastric carcinoma group increased compared with that in normal group（P〈0.05）. The expression of CD4＋CD25＋regulatory T cells in peripheral blood of patients with advanced gastric carcinoma, gastric carcinoma in T3＋T4 and gastric carcinoma with lymph node metastasis was higher than that in patients with early gastric carcinoma, gastric carcinoma in T1＋T2 and gastric carci noma without lymph node metastasis respectively（P〈0.05）. No significant difference was noticed in the expression of Th17 cells in patients with gastric carcinoma in different stages, depth of infiltration or with lymph node metastasis（P〈0.05）. Conclusion The expression of both CD4＋CD25＋regulatory T cells and Th17 cells in peripheral blood of patients with gastric carcinoma increased significantly. It was also suggested the progression of gastric carcinoma was associated with CD4＋CD25＋regulatory T cells, but not associated with Th17 cells statistically.