番荔枝花发育不同时期的数字基因表达谱分析

Digital Gene Expression Analysis of Different Flower Developmental Stages in Sugar Apple(Annona squamosa L.)

采用Illunima HiSeq^(TM) 2500测序技术对不同发育时期的番荔枝花进行从头组装和注释分析,以期得到和挖掘更多与番荔枝花发育相关的基因数据和功能.测序结果表明:番荔枝花在不同发育时期中4个开花调控途径均已启动.转录组测序共获得107 197 488条clean reads,包含13 399 686 000个核苷酸序列信息.对原始数据进行组装,获得71 948条Unigenes序列信息,平均长度为825.4bp,将获得的Unigene序列与已知数据库进行比对,共有24 911条Unigene被注释到.其中,8 682条Unigene被注释到COG,COG功能涉及到各种类的生命活动,整体功能类的基因最多,有2 403条;6 837条Unigene匹配到KEGG数据库,Unigene参与的番荔枝代谢通路可以分为124类.

In order to obtain the genetic datum and functions of genes in sugar apple,the study was carried on the transcriptome analysis of sugar Apple in different flower developmental stages.In this study,RNA-Seq generated 107 197 488 sequence reads encompassing 13 399 686 000 total nucleotides（nt）.All high-quality reads were assembled into 71 948 unigene with a mean size of 825.4 bp.A total of 24 911 unigenes were annotated using public protein databases.A total of 8 682 unigenes were annotated to Clusters of Orthologous groups.The COG functional classifications involved most of the life activities.The cluster for＂generalfunction prediction only＂represented the largest group,with 2 403 genes.Functional annotation against Kyoto Encyclopedia of Genes and Genomes pathway database identified 6 837 unigenes which were mapped to 128 pathways.