番茄部分WRKY基因非生物胁迫表达和SlWRKY50基因沉默分析

Partial WRKY genes expression under non-biological stress and analysis of SlWRKY50 gene silencing in tomato

前期研究已完成番茄WRKY转录因子家族分析,发现番茄WRKYⅡa和Ⅱb亚族基因多与抗逆相关,因此文章选取6个WRKYⅡa和Ⅱb亚族基因Sl WRKY13、Sl WRKY24、Sl WRKY31、Sl WRKY50、Sl WRKY62、Sl WRKY63,运用q RT-PCR分析方法,分析逆境胁迫下表达模式。结果表明,Sl WRKY24干旱、盐、低温胁迫下表达受抑制,另外5个基因在三种胁迫处理中,除干旱胁迫处理Sl WRKY50基因表达量下降,其余均不同程度上调表达。低温胁迫处理下Sl WRKY50基因表达量与对照相比表现极显著差异。Sl WRKY50基因沉默分析结果表明,Sl WRKY50基因沉默后,下调Sl WRKY50基因表达,干旱、高盐胁迫下植株叶片Pro、SOD、MAD含量水平与对照相比变化较小,低温胁迫下番茄植株叶片Pro和SOD含量低于对照,MAD提高含量,植株对低温逆境胁迫耐受能力下降。研究为进一步探讨WRKY基因家族功能提供参考及理论依据。

This research selected six WRKY gene including Sl WRKY13, Sl WRKY24, Sl WRKY31,Sl WRKY50, Sl WRKY62, Sl WRKY63 through early studies, and was used q RT-PCR analysis method to analyze the expression differences in stress treatments. Sl WRKY24 expression was inhibited under stress of drought, salt and low temperature.The other five genes were treated in three kinds of stress treatments, in general, the expression quantity was increased with time, reached the maximum value and then decreased,except the drought stress treatment of Sl WRKY50 gene, its expression was decreased. Under low temperature stress, the expression of Sl WRKY50 gene was significantly different from that of control. Finally,We analyzed Sl WRKY50 gene by gene silencing method, the results showed when the gene was silencing,the contents of Pro, SOD and MAD were a little change compared with control under drought and high salt stress in plant leaves, and under low temperature stress in the tomato leaves the content of Pro and SOD was reduced compared with control, but improved the content of MAD. It suggested that Sl WRKY50 gene was involved in response to low temperature stress.This study provided reference and theoretical basis for further studying on the function of WRKY gene family.