液相色谱法测定保健酒中的西地那非和咖啡因

Determination of sildenafil and caffeine in health wine by liquid chromatography

建立了液相色谱法测定保健酒中西地那非和咖非因的含量。样品采用C18色谱柱梯度洗脱,波长为280nm,流动相为乙腈–0.1%磷酸溶液,柱温为30℃,采用二极管阵列检测器检测。西地那非和咖非因的质量浓度在5～200μg/mL范围内与色谱峰面积呈良好的线性关系,相关系数大于0.999,西地那非的检出限为0.32 mg/kg,咖啡因的检出限为0.17 mg/kg。测定结果的相对标准偏差为1.0%～3.0%(n=6),样品加标回收率为94.2%～98.7%。该方法操作简单、快速,结果准确,适用于保健酒中西地那非和咖啡因的同时测定。

A method for the determination of sildenafil and caffeine in health wine by liquid chromatography was established. The sample was eluted with C18 column gradient, the wavelength was 280 nm, the mobile phase was acetonitrile–0.1% phosphoric acid solution, the column temperature was 30℃, and the diode array detector was used. The mass concentration of sildenafil and caffeine had good linear relationship with the chromatographic peak area in the range of 5–200 μg/mL, the correlation coefficients were more than 0.999. The detection limit was 0.32 mg/kg for sildenafil, and 0.17 mg/kg for caffeine. The relative standard deviation of the detection results was 1.0%–3.0% （n=6）, and the recoveries of sample were 94.2%–98.7%. The method is simple, rapid and accurate. It is suitable for simultaneous determination of sildenafil and caffeine in health wine.