CQMUH-011对巨噬细胞增殖与吞噬功能的影响

Effects of CQMUH-011 on Proliferation and Phagocytosis of Macrophages

目的观察CQMUH-011对巨噬细胞增殖及吞噬功能的影响。方法采用噻唑蓝(MTT)法检测不同浓度CQMUH-011预处理不同时间对脂多糖(LPS)诱导的巨噬细胞(RAW264.7)增殖的影响;酶联免疫吸附法(ELISA)检测CQMUH-0011对LPS刺激不同时间后RAW264.7细胞分泌肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)的影响;碳廓清实验和腹腔巨噬细胞吞噬实验检测CQMUH-011对小鼠巨噬细胞吞噬功能影响。结果 1μmol·m L^(-1)CQMUH-011预处理24 h,对LPS刺激的RAW264.7细胞增殖的抑制作用最显著(P<0.01),对LPS刺激不同时间后RAW264.7细胞分泌TNF-α、IL-1β均有显著降低作用(P<0.01)。CQMUH-011 225μg·kg^(-1)对小鼠碳廓清速率和腹腔巨噬细胞吞噬功能均有显著抑制作用(P<0.01)。结论 CQMUH-011对巨噬细胞增殖、炎症因子产生及吞噬功能均有抑制作用。

Objective To investigate the effects of CQMUH-011 on proliferation and phagocytosis of macrophages.Methods MTT assay was used for detecting the effects of different concentrations of CQMUH-011 pretreatment for different time on the proliferation of macrophages （RAW264.7） induced by lipopolysaccharide （LPS）.Enzyme-linked immunosorbent assay （ELISA） was used for examining the effects of CQMUH-0011 on the secretion of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） induced by LPS.Carbon clearance experiment and peritoneal macrophages phagocytosis experiments were used for detecting the effects of CQMUH-011 on phagocytosis of macrophages in mice.Results The most significant inhibitory effect of CQMUH-011 on the proliferation of LPS-induced RAW264.7 cells was at the concentration of 1 μmol·mL -1 and pretreatment for 24 h （ P 〈0.01）, and the levels of inflammatory cytokines such as TNF-α and IL-1β induced by LPS at different time points were significantly inhibited （ P 〈0.01）.Moreover, 225 μg·kg -1 of CQMUH-011 significantly inhibited the carbon clearance rate and phagocytosis of macrophages （ P 〈0.01）.Conclusion CQMUH-011 inhibits the proliferation, inflammatory factor production and phagocytosis of macrophages.