镇肝熄风汤对自发性高血压大鼠主动脉弓血管组织形态学的影响

Effect of Zhengan Xifeng Decoction on Vascular Tissue Morphology of Aortic Arch in Spontaneously Hypertensive Rats

目的研究不同剂量镇肝熄风汤对自发性高血压大鼠(SHR)主动脉弓血管组织形态学的影响。方法将50只24周龄SHR随机分为模型对照组,镇肝熄风汤小、中、大剂量组,复方罗布麻组,每组10只,以同源雄性京都威斯特大鼠(WKY)为正常对照组;模型对照组和正常对照组灌服0.9%氯化钠溶液4 m L·d^(-1),镇肝熄风汤小、中、大剂量组分别灌服镇肝熄风汤13.5,27,54 g·kg^(-1)·d^(-1),复方罗布麻组灌服复方罗布麻混悬液54 g·kg^(-1)·d^(-1),50 d后苏木精-伊红染色法观察主动脉弓组织病理学形态变化。结果与模型对照组比较,镇肝熄风汤中、大剂量组和复方罗布麻组主动脉弓血管壁厚度明显减小(P<0.01);与正常对照组比较,镇肝熄风汤大剂量组、复方罗布麻组主动脉弓血管壁厚度无明显变化;与复方罗布麻组比较,镇肝熄风汤大剂量组主动脉弓血管壁厚度无明显变化(P>0.05)。结论镇肝熄风汤能改善SHR血管壁病理结构,减少高血压对血管壁的破坏,减轻血管壁增厚。

Objective To study effect of different doses of Zhengan xifeng decoction on vascular tissue morphology of aortic arch in spontaneously hypertensive rats （SHR）.Methods A total of 50 male SHR,24 weeks old,were randomly divided into model control group,low,medium,high dose of Zhengan xifeng decoction groups and compound apocynum group,10 in each group.Ten homologous male rats （WKY） served as normal control group.The model control group and normal control group were fed with 4 mL·d ^-1 0.9%sodium chloride solution.Low, midium and high dose groups were fed with Zhengan xifeng decoction （13.5,27,54 g·kg ^-1 ·d^ -1 ,respectively）.Compound apocynum group was fed with 54 g·kg ^-1 ·d ^-1 compound apocynum suspension.After 50 days,the histopathological changes of the aortic arch were observed with HE staining.Results Compared with the model control group,the wall diameter of the aortic arch was significantly reduced in the middle dose group,high dose group,and compound apocynum group （ P 〈0.01）.There were no significant difference in the wall diameter of the aortic arch between the high dose group,the compound apocynum group and the normal control group （ P 〉0.05）.There was no significant difference in the wall diameter of the aortic arch between the high dose group and the compound apocynum group （ P 〉0.05）.Conclusion Zhengan xifeng decoction can improve the pathological structure of SHR vascular wall,reduce the injury of blood pressure to vessel wall,and reduce the thickening of vessel wall.