高效液相色谱法测定复方赖氨酸颗粒中盐酸赖氨酸含量

Determination of Lysine Hydrochloride in Compound Lysine Granules by HPLC

目的建立高效液相色谱(HPLC)法测定复方赖氨酸颗粒中赖氨酸的含量。方法采用氨基柱(150 mm×4.6 mm,5μm),以0.05 mol·L^(-1)磷酸二氢铵溶液为流动相,流速为0.5 m L·min^(-1),柱温为25℃,检测波长为205 nm。结果赖氨酸的线性范围为0.063~2.521 mg·m L^(-1)(R^2=1.000),平均回收率(n=9)为99.2%。结论该方法操作简便,结果准确,可用于复方赖氨酸颗粒中赖氨酸的含量测定。

Objective To establish a high performance liquid chromatography（HPLC）method for the determination of lysine hydrochloride in compound lysine granules.MethodsThe determination was performed on NH2column（150 mm×4.6 mm,5μm）with mobile phase consisted of 0.05 mol·L^-1ammonium dihydrogen phosphate at a flow rate of 0.5 m L·min^-1.The column temperature was 25℃,and the detection wavelength was set at 205 nm.ResultsThe linear range of them was0.063-2.521 mg·m L^-1（R^2=1.000）.The average recovery（n=9）was 99.2%.ConclusionThe method is simple and accurate,and can be used for assay of lysine in compound lysine granules.