阿尔泰金莲花总黄酮及其荭草素2″-O-β-L-半乳糖苷的提取工艺

Optimization of Extracting Technology of Total Flavonoids and Orientin-2″-O-β-Lalactopyranosylide from Trollius altaicus

目的研究阿尔泰金莲花总黄酮及其荭草素2″-O-β-L-半乳糖苷(OGA)的提取工艺。方法在含量测定基础上,以总黄酮和OGA含量以及DPPH自由基清除能力为评价指标,以乙醇浓度、料液比、提取时间和提取次数为因素,通过L_9(3~4)正交实验设计优选阿尔泰金莲花总黄酮及其OGA的提取工艺。结果阿尔泰金莲花总黄酮及其OGA的最佳提取工艺条件为:乙醇体积分数70%,料液比1∶30(g·m L^(-1)),提取时间1.5 h,提取2次;该工艺可以获得高含量的总黄酮[(170.53±3.30)mg·g^(-1)]和OGA[(29.02±0.46)mg·g^(-1)]。抗氧化活性随提取物中总黄酮和OGA含量的增大而增大。结论该提取工艺操作简便,可为阿尔泰金莲花的开发利用提供参考。

ObjectiveTo study extraction technique of orientin-2″-O-β-L-galactopyranosylide（OGA）and total flavonoids from Trollius altaicus.MethodsBased on the determination of OGA and total flavonoids content as well as evaluation of antioxidant activities,the extraction of flavone from T.altaicus was optimized by orthogonal experiment with these indexes.The ethanol concentration,solid to liquid ratio,extraction time and frequency were selected as affecting factors.Extraction technique of orientin-2″-O-β-L-galactopyranosylide（OGA）and total flavonoids from Trollius altaicus was optimized by L9（3-4）orthogonal experimental design.ResultsThe optimum extraction technique was shown as follows：the ethanol concentration70%,solid-liquid ratio 1∶30（g·m L^-1）and the extraction time 1.5 h,frequency 2 times.The content of OGA and total flavonoids from T altaicus were（29.02±0.46）mg·g^-1and（170.53±3.30）mg·g^-1respectively under the optimal condition.The antioxidant activities were improved with the increase of total flavonoids and OGA contents in the extracts.ConclusionThe optimum extraction process is suitable for industrial production due to its simple manipulation,and it can provide essential data for the development and utilization of T.altaicus.