冠突散囊菌利用不同原料产洛伐他汀的条件优化

Optimization of Producing Lovastatin by Eurotium cristatum with Different Materials

本研究以甜菜粉、甜菜粕、甜叶菊、甘草渣及麸皮为原料用冠突散囊菌发酵产洛伐他汀,分别考察了这些原料和不同因素对洛伐他汀产量的影响.甜菜粉作为培养基时洛伐他汀的含量最高为95 mg/kg.添加0.15%维生素B_2以甜菜粉为原料产洛伐他汀的量最高(134 mg/kg),与不添加维生素B_2相比产量提高了41%,以添加量(A)、发酵温度(B)、发酵时间(C)及接种量(D)4个因素进行正交试验.结果表明,各因素对洛伐他汀产量的影响大小依次为:添加量(A)>接种量(D)>发酵时间(C)>发酵温度(B).最优组合为A_2B_3C_1D_2,在此条件下洛伐他汀产量为146.4 mg/kg.

In this work,it is studied on the production of lovastatin by Eurotium cristatum from beet powder,beet pulp, stvia rebaudiana,glycyrrhiza residue and wheat bran as raw materials,and the effects of these materials and different factors on the yield of lovastatin were investigated. Eurotium cristatum which cultivated by beet powder had the highest lovastatin level（95 mg/kg）. Vitamin B2（with a addition of 0.15%）could make the production of lovastatin significantly raise 41%（134 mg/kg）compared with no precursors. Choose 4 factors to conduct orthogonal test,the results showed the influence order of all factors are,precursors concentration（A）〉inoculum concentration（D）〉fermentation time（C）〉fermentation temperature（B）. The optimum extraction technology is A2B3C1D2,and the production of lovastatin could reach 146.4 mg/kg.