响应曲面法优化枸骨果实多糖提取工艺

Optimization of Extraction Technology of Polysaccharide from Fruits of Ilex cornuta by Response Surface Methodology

为探讨枸骨果实多糖的最佳提取工艺,在单因素试验的基础上,以多糖提取率为响应值,通过Box-Bekxken实验设计和响应面分析建立二次回归模型方程,对影响多糖提取率的3个主要因素:液料比、提取温度、提取时间进行优化,结果显示:枸骨果实多糖提取的最佳工艺条件是提取温度80℃、提取时间1.5 h、液料比150∶1(m L/g)。该工艺参数条件下的枸骨果实多糖的提取率达到28.84%。

In order to optimize extraction conditions of polysaccharides from fruits of Ilex cornuta,On the basis of single factor experiment of liquid to solid ratio,extraction time and temperature,we used Box-Behnken central composite design and response surface methodology to built quadratic response surface regression model and the liquid to solid ratio,extraction time and temperature were optimized and arranged. The optimal extraction parameters were described as follows：the extraction temperature was 80 ℃,the extraction time was1.5 h,and the liquid material ration was 150 ∶ 1（m L/g）. Under the conditions of the process parameters,the extraction rate of polysaccharides from Fruits of Ilex cornuta was 28.84 %.