热灌注化疗联合全身化疗对晚期膀胱癌病灶中恶性分子表达和分泌的影响

Effect of hyperthermia perfusion chemotherapy combined with systemic chemotherapy on the expression and secretion of malignant molecules in advanced bladder cancer

目的:研究热灌注化疗联合全身化疗对晚期膀胱癌病灶中恶性分子表达和分泌的影响。方法:选择在公安县人民医院诊断为晚期膀胱癌的患者并随机分为两组,实验组接受热灌注化疗联合全身化疗、对照组接受常规灌注化疗联合全身化疗。化疗前及化疗后3个月,测定病灶内增殖侵袭基因的表达量及尿液中细胞因子的分泌量。结果:组内化疗后病灶内Livin、Bcl-2、TRAP1、MMP9的mRNA表达量以及尿液中VEGF、TGF-β1、MCP-1、CEACAM1的分泌量低于化疗前,病灶内Bad、LRIG3、Beclin1、KLF4、CHD13、E-cadherin的mRNA表达量以及尿液中IFN-γ、IL-2的分泌量高于化疗前且实验组患者病灶内Livin、Bcl-2、TRAP1、MMP9的mRNA表达量以及尿液中VEGF、TGF-β1、MCP-1、CEACAM1的分泌量显著低于对照组,病灶内Bad、LRIG3、Beclin1、KLF4、CHD13、E-cadherin的mRNA表达量以及尿液中IFN-γ、IL-2的分泌量显著高于对照组。结论:热灌注化疗联合全身化疗能够较常规灌注化疗联合全身化疗更为有效地调节晚期膀胱癌病灶中恶性分子的表达及分泌。

Objective：To study the effect of hyperthermia perfusion chemotherapy combined with systemic chemotherapy on the expression and secretion of malignant molecules in advanced bladder cancer.Methods：Patients who were diagnosed with advanced bladder cancer in Gongan County People＇s Hospital between March 2015 and December 2017 were chosen and randomly divided into two groups：experimental group accepted hyperthermia perfusion chemotherapy combined with systemic chemotherapy,and control group accepted routine infusion chemotherapy combined with systemic chemotherapy.The expression levels of proliferation and invasion genes in the lesions as well as the secretion of cytokines in the urine were measured before chemotherapy and 3 months after chemotherapy.Results：Livin,Bcl-2,TRAP1 and MMP9 mRNA expression in lesions as well as VEGF,TGF-β1,MCP-1 and CEACAM1 secretion in urine of both groups after chemotherapy were lower than those before chemotherapy whereas Bad,LRIG3,Beclin1,KLF4,CHD13 and E-cadherin mRNA expression in lesions as well as IFN-γand IL-2 secretion in urine were higher than those before chemotherapy,and Livin,Bcl-2,TRAP1 and MMP9 mRNA expression in lesions as well as VEGF,TGF-β1,MCP-1 and CEACAM1 secretion in urine of experimental group were significantly lower than those of control group whereas Bad,LRIG3,Beclin1,KLF4,CHD13 and E-cadherin mRNA expression in lesions as well as IFN-γand IL-2 secretion in urine were significantly higher than those of control group（P〈0.05）.Conclusion：Hyperthermia perfusion chemotherapy combined with systemic chemotherapy can be more effective than routine infusion chemotherapy combined with systemic chemotherapy to regulate the expression and secretion of malignant molecules in advanced bladder cancer.