基于BSA法开发CAPS标记定位甜瓜果面沟相关基因研究

Study on develop CAPS markers to locate genes related to melon fruit surface groove based on BSA method

试验以无果面沟甜瓜品系M4-5为母本,有果面沟甜瓜品系M1-15为父本,配制杂交组合获得F2代群体,作果面沟性状遗传分析,发现甜瓜果面沟性状为显性遗传,受一对基因控制。通过果实性状相关性分析,果面沟性状与裂果呈显著负相关。利用群体分离分析法(BSA)筛选得到甜瓜果面沟基因位于第11号染色体后半段,以双亲材料基因重测序为基础,在定位区域上开发30对引物,在等区段分割处选择15对具有多态性引物。利用15对引物标记F2代群体,构建分子遗传图谱,该图谱全长181.87 c M。定位到一个距离为3.6 c M的控制果面沟位点,通过加密最终得到一个距离为1.1 c M位点,两个与该位点紧密连锁标记分别为M11-01和M11-51。利用100株甜瓜自然群体材料分析2个与果面沟紧密连锁标记,分子数据与田间数据吻合率分别为74.67%和75.99%。

Using the melon line M4-5 that had no surface groove as the female parent, the melon line M1-15 that had surface groove as the male parent, to obtain the hybrid population F2. Surveying the genetic regularity of the surface groove, it could be surface groove was a dominant character and controlled by a pair of genes. According to the correlation analysis of fruit traits, the fruit mulch trait was negatively correlated with dehiscent fruit. Using the BSA method to determine the surface groove gene on chromosome 11. Based on genomic resequencing data for parent materials, developing 30 pairs of primers on chromosome and selecting 15 pairs of polymorphic primers in equal segments. Using these 15 pairs of primers to label F2 populations to construct genetic linkage maps that length was 181.87 cM. Initially, obtaining a genetic locus with a distance of 3.6 cM controlling the melon surface groove, and finally obtaining a genetic locus with a distance of 1.1 cM through more detailed process. Two closely linked markers were obtained, which were M11-01 and M11-51, respectively. Using the 100 melon natural populations, the markers closely connected with the fruit surface groove were analyzed, and the molecular data were consistent with the field data. The rates were 74.67% and 75.99%, respectively.