三株生防酵母菌对Botrytis cinerea防治效果研究

Study on control effect of three biocontrol yeasts against Botrytis cinerea

文章研究三株生防酵母菌Cryptococcus albidus 63(Ca63)、Cryptococcus albidus 64(Ca64)和Candida parapsilosis yett1006对番茄灰霉病病原菌B.cinerea t08016b防治效果。番茄果实贮藏试验表明,三株生防酵母菌均有贮藏防腐效果,其中C.albidus 64防腐效果最好,好果率达45.83%。生防酵母菌对番茄灰霉病离体果实防治效果试验表明,三株生防酵母菌均可抑制番茄灰霉病,其中C.albidus 64防治效果最好,单一菌液处理对番茄灰霉病菌防治效果达95.83%,同时接种生防酵母菌和灰霉病菌对番茄灰霉病菌防治效果达69.57%。三株生防酵母菌对番茄灰霉病诱导抗性试验结果表明,三株生防酵母菌均可诱导番茄果实中防御相关酶如多酚氧化酶(PPO)、过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性且活性氧(H2O2、O2-)含量产生明显提高,可增强番茄对灰霉病抗病能力。

In this study, the control effects of three biocontrol yeasts Cryptococcus albidus 63（Ca63）,Cryptococcus albidus 64（Ca64） and Candida parapsilosis yett1006 against Botrytis cinerea were investigated. The storage test of tomato fruit showed that three biocontrol yeasts had anti-corrosion effect.C.albidus 64 had the best anti-corrosion effect and the good fruit rate was 45.83%. The control effect of biocontrol yeasts on isolated fruit of Botrytis cinerea was tested and it was shown that the three biocontrol yeasts could inhibit Botrytis cinerea. C. albidus 64 had the best control effect, with 95.83% of the control effect against Botrytis cinerea treated with a single bacterial solution, and 69.57% of the control effect against Botrytis cinerea inoculated with biocontrol yeast and Botrytis cinerea simultaneously. The results showed that the three biocontrol yeasts could induce the increase of defense-related enzyme activities, such as PPO,POD and PAL, significantly increase active oxygen contents, such as H_2O_2 and O_2^-, and enhance the diseaseresistance of tomato against Botrytis cinerea.