摘要
采用可逆加成-断裂链转移(Reversible addition fragmentation chain transfer,RAFT)分子印迹技术,以内分泌干扰物17β-雌二醇(17β-estradiol,17β-E_2)作为模板分子,以甲基丙烯酸(Methacrylic acid,MAA)为功能单体,二乙烯基苯(Divinyl benzene,DVB)为交联剂,Fe_3O_4/MnO_2掺杂的石墨烯(RGO)为载体,成功制备了Fe_3O_4/MnO_2掺杂石墨烯基分子印迹杂化材料(Fe_3O_4/MnO_2-MIP@RGO),构建了基于Fe_3O_4/MnO_2-MIP@RGO修饰电极的分子印迹电化学传感器。研究结果表明,Fe_3O_4/MnO_2-MIP@RGO修饰电极对17β-E_2的线性响应范围为4.0 nmol/L^0.8μmol/L(R=0.9852),检出限为47.2 pmol/L(3σ),相对标准偏差(RSD)为2.1%~2.5%。本研究为水环境中痕量17β-E_2的特异性识别检测提供了一种简单、高效、经济的分析方法。
In this paper, Fe3O4 / MnO2 doped graphene molecularly imprinted hybrid material (Fe3O4 / MnO2 -MIP@ RGO) was successfully synthesized via reversible addition fragmentation chain transfer ( RAFT)molecularly imprinting technique by using methacrylic acid as functional monomer, divinylbenzene as cross-linker, Fe3O4 / MnO2 @ RGO as carrier, and 17β-estradiol ( 17β-E2 ) as template molecule. A novelmolecularly imprinting electrochemical sensor by using Fe3O4 / MnO2 -MIP @ RGO modified electrode wasconstructed to specifically detect trace 17β-E2 in water. The experimental results showed that the Fe3O4 /MnO2 -MIP@ RGO electrochemical sensor exhibited rapid and linear current response to 17β-E2 in watersamples with a linear range of 4 nmol / L to 0. 8 滋mol / L (R = 0. 9852), the detection limit was 47. 2 pmol / L(3滓) and the relative standard deviation (RSD) was from 2. 1% to 2. 5%. This study provides a simple andefficient, economical and reliable method for the monitoring of 17β-estradiol in the complex waterenvironment.
作者
李颖
徐文凯
李苹
朱小雪
黄艳凤
张纪梅
LI Ying;XU Wen-Kai;LI Ping;ZHU Xiao-Xue;HUANG Yan-Feng*;ZHANG Ji-Mei(School of Environmental and Chemical Engineering,Tianjin Polytechnic University,State Key Laboratory of Hollow Fiber Membrane Materials and Membrane Process,Tianjin 300387,China;School of Materials Science and Engineering,Harbin Institute of Technology,Harbin 150001,China)
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2018年第7期1047-1054,共8页
Chinese Journal of Analytical Chemistry
基金
本文系国家自然科学基金项目(Nos.21106101,21475095)、天津市自然科学基金项目(No.13JCQNJC06300)和中国博士后基金项目(No.2013M540281)资助
关键词
可逆加成-断裂链转移分子印迹技术
石墨烯
电化学传感器
金属氧化物
17Β-雌二醇
Reversible addition fragmentation chain transfer molecularly imprinting technique
Reducedgraphene oxide
Electrochemical sensor
Metal oxide
17茁-Estradiol