Fe3O4/MnO2掺杂石墨烯基分子印迹杂化材料用于水环境中17β-雌二醇的电化学传感检测

Molecularly Imprinted Polymer Electrochemical Sensor Based on Fe_3O_4/MnO_2 Doped Graphene Composites for Determination of 17β-Estradiol in Aquatic Environment

采用可逆加成-断裂链转移(Reversible addition fragmentation chain transfer,RAFT)分子印迹技术,以内分泌干扰物17β-雌二醇(17β-estradiol,17β-E_2)作为模板分子,以甲基丙烯酸(Methacrylic acid,MAA)为功能单体,二乙烯基苯(Divinyl benzene,DVB)为交联剂,Fe_3O_4/MnO_2掺杂的石墨烯(RGO)为载体,成功制备了Fe_3O_4/MnO_2掺杂石墨烯基分子印迹杂化材料(Fe_3O_4/MnO_2-MIP@RGO),构建了基于Fe_3O_4/MnO_2-MIP@RGO修饰电极的分子印迹电化学传感器。研究结果表明,Fe_3O_4/MnO_2-MIP@RGO修饰电极对17β-E_2的线性响应范围为4.0 nmol/L^0.8μmol/L(R=0.9852),检出限为47.2 pmol/L(3σ),相对标准偏差(RSD)为2.1%~2.5%。本研究为水环境中痕量17β-E_2的特异性识别检测提供了一种简单、高效、经济的分析方法。

In this paper, Fe3O4 / MnO2 doped graphene molecularly imprinted hybrid material （Fe3O4 / MnO2 -MIP@ RGO） was successfully synthesized via reversible addition fragmentation chain transfer （ RAFT）molecularly imprinting technique by using methacrylic acid as functional monomer, divinylbenzene as cross-linker, Fe3O4 / MnO2 @ RGO as carrier, and 17β-estradiol （ 17β-E2 ） as template molecule. A novelmolecularly imprinting electrochemical sensor by using Fe3O4 / MnO2 -MIP @ RGO modified electrode wasconstructed to specifically detect trace 17β-E2 in water. The experimental results showed that the Fe3O4 /MnO2 -MIP@ RGO electrochemical sensor exhibited rapid and linear current response to 17β-E2 in watersamples with a linear range of 4 nmol / L to 0. 8 滋mol / L （R = 0. 9852）, the detection limit was 47. 2 pmol / L（3滓） and the relative standard deviation （RSD） was from 2. 1% to 2. 5%. This study provides a simple andefficient, economical and reliable method for the monitoring of 17β-estradiol in the complex waterenvironment.