羟基磷灰石纳米线/还原氧化石墨烯/纳米金复合材料基传感器在抗坏血酸检测中的应用研究

Potential of ascorbic acid sensitive sensor fabricated using hydroxyapatite nanowire/reduced graphene oxide/gold nanoparticle

羟基磷灰石纳米线具有比表面积大、吸附性强,生物相容性好的优点,利用石墨烯优异的导电性与羟基磷灰石复合制成纳米复合材料,该复合材料在电化学领域得到了越来越广泛的应用。利用水热法一步制备出羟基磷灰石纳米线/还原氧化石墨烯/纳米金复合材料,并用该复合材料修饰的玻碳电极作为工作电极制造出抗坏血酸氧化酶传感器,该传感器对抗坏血酸的电化学性能结果表明:纳米复合材料修饰的工作电极对抗坏血酸有优异的电化学活性,峰值电流与抗坏血酸浓度呈现良好的线性关系;抗坏血酸氧化酶传感器灵敏度为1.5949×10-2 A/moL,线性检测范围为3.90×10-4~3.60×10-2 mol/L(R2=0.99845),最低检测限为3.39×10-6 mol/L(S/N=3)。实验结果表明该抗坏血酸氧化酶传感器具有灵敏度好,线性检测范围宽,最低检测限小的优点,在对抗坏血酸检测领域具有广泛的应用前景。

Hydroxyapatite nanowires have the advantages of large specific surface area,strong adsorbability and good biocompatibility.Graphenehave the advantages ofexcellent electrical conductivity,so the nanocomposite made by the two materials has been used in electrochemical field widely.In this study,a ternary nanocomposite consisting of gold nanoparticles（AuNPs）,hydroxyapatite（HAP）nanowires,and reduced graphene oxide（rGO）is synthesized by a simple one-step hydrothermal method,which is used to modify glassy carbon electrode（GCE）for detectingascorbic acid.Experimental results show that the GCE modified by the nanocomposites have superior electrocatalytic activities for ascorbic acid.The peak current intensities HAP-rGO/AuNPs sensing system linearly increase as the ascorbic acid concentration increasessubstantially at a range of 3.90×10^-4 to 3.60×10^-2 mol/L（R2=0.99845）,with a analytical sensitivity of 1.594 9×10^-2 A/moL and detection limit of 3.39×10^-6 mol/L（S/N=3）.The experimental results show that this ascorbate oxidase sensor has the advantages of good sensitivity,wide linear detection range and low detection limit and it has broad application prospects in the field of detectascorbic acid.