磷灰石涂层用于调控神经细胞的生物学行为

Biological behavior of apatite coating used to regulate nerve cell

利用仿生矿化法在钛合金表面制备磷灰石(Apatite)涂层,并通过仿生共沉积的方式在该涂层表面固定层粘连蛋白(Laminin,LN),在钛合金表面构建磷灰石-LN功能性复合涂层。通过场发射扫描电子显微镜(FESEM)和X射线光电子能谱仪(XPS)对磷灰石与磷灰石-LN复合涂层进行表征,发现制备的磷灰石涂层呈现均一的多孔片层状结构,对LN分子有良好的固定能力。磷灰石-LN复合涂层的LN释放实验结果表明:构建的磷灰石-LN复合涂层中的LN分子可从涂层表面缓慢而持续地释放。神经细胞PC12与磷灰石、磷灰石-LN复合涂层的联合体外培养实验结果表明:磷灰石、磷灰石-LN复合涂层具有良好的生物相容性,且磷灰石-LN复合涂层具有一定的促进神经细胞贴附和增殖的作用。制备的磷灰石涂层作为仿生共沉积生物活性分子的理想载体,在调控神经细胞生长以及周围神经损伤的修复领域有着良好的应用前景。

Apatite coating was prepared on titanium alloy surface with biomimetic mineralization method.Laminin（LN）was fixed on the coating through bionic codeposition.Apatite-LN functional composite coating was constructed on the titanium alloy surface.Apatite and apatite-LN coating were characterized by field emission scan electron microscope（FE-SEM）and X-ray photoelectron spectroscopy（XPS）.The results showed that the prepared apatite coating presented uniform porous lamella structure,and had good fixation ability for LN molecule.The results of release test of apatite-LN coating suggested that,LN molecule in f apatite-LN coating could release slowly and continuously from the coating surface.Combined in-vitro culture experiment of nerve cell PC12,apatite and apatite-LN coating shows that,apatite and apatite-LN coating had good biocompatibility,and apatite-LN coating had the function of promoting attachment and proliferation of nerve cell.The prepared apatite coating as the ideal carrier of bionic codeposition bioactive molecule has the excellent application prospect in regulating nerve cell growth and repairing the peripheral nerve injury.