VgDGAT1A转基因大豆高代材料的表型分析

Phenotypic analysis of VgDGAT1A-transgenic soybean seeds

[目的]二酰甘油酰基转移酶(DGAT)是植物三酰甘油(TAG)合成的最后一步限速酶,对油脂积累至关重要,可用于提高大豆(Glycine max)种子油含量。[方法]本文将来自富油植物斑鸠菊(Vernonia galamensis)发育种子的VgDGAT1A基因转入大豆品种Jack,进一步培育获得VgDGAT1A转基因大豆高代品系。通过qRT-PCR检测VgDGAT1A基因在大豆种子发育中期的表达情况及其引起的脂肪酸含量变化。[结果]连续多代跟踪检测表明,这些大豆品系中VgDGAT1A基因稳定整入染色体,并有效表达。与野生型大豆(Jack)相比,转基因大豆种子油脂含量平均提高5.1%,没有其它不良表型。[结论]VgDGAT1A基因确实能够在大豆种子发育过程中促进油脂合成累积,从而提高大豆种子的总体含油量。这些富油转基因品系可进一步用于培育高油大豆品种,以及大豆种子油脂合成积累及其调控机制的研究。

[Objective]Diacylglycerol acyltransferase（DGAT）is the rate-limiting enzyme in the final step of plant triacylglycerol（TAG）synthesis,which plays a key role in the accumulation of total fatty acids of soybean seeds and can be modified to increase the oil content of soybean seed.[Methods]The VgDGAT1 Agene extracted from oil-rich seed of Vernonia galamensis was transferred into Jack soybean variety,and transgenic soybean high-generation line was obtained after further cultivation.Expression of VgDGAT1 Agene and the variation of fatty acid content in transgenic soybeans during mid-period of soybean seed mature process were measured by qRT-PCR.[Results]Several consecutive generations were tracked down,and tracing assays showed that the VgDGAT1 Agene in these soybean lines was stably integrated into the chromosome and expressed efficiently.Compared to wild-type soybean（Jack）,the content of oil in transgenic soybean seeds was increased by 5.1% on average,and no undesirable phenotypes were observed.[Conclusion]Studies indicated that the VgDGAT1 Agene could improve the synthesis and accumulation of lipid in developmental process of soybean seed,thus increase the overall oil content of soybean seeds.These oil-rich transgenic lines can be used to cultivate high-oil soybean varieties,and used to study the accumulation and regulation of soybean oil synthesis and regulation.